168,579 results

1. pLNHA-C1-HsNot8-D40AE42A_U

   Plasmid

   #147647

   Purpose

   Mammalian Expression of HsNot8-D40AE42A

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsNot8-D40AE42A (CNOT8 Human)
   

   Expression

   Mammalian

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEP200-Lenti-TRE3G-Flag-GluN2A-WPRE-pA

   Plasmid

   #164724

   Purpose

   pLenti vector backbone designed to express Flag-GluN2A from a TRE3G promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2A
   

   Use

   Lentiviral

   Tags

   Flag

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJEP213-Lenti-1.1Synapsin P-GFP-WPRE-pA

   Plasmid

   #62930

   Purpose

   pLenti vector backbone designed to express GFP from a 1.1Synapsin promoter .

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   Green Fluorescent protein (GFP)
   

   Use

   Lentiviral

   Promoter

   1.1Synapsin and 1.3αCaMKII

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCD1E

   Plasmid

   #102956

   Purpose

   gene cloning of human CD1E

   Depositor

   Gennaro De Libero, Lucia Mori

   Article

   Facciotti et al Proc Natl Acad Sci U S A. 2011 Aug 23;10

   Insert

   CD1E cDNA (CD1E Human)
   

   Use

   Cloning

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHMR1B

   Plasmid

   #102958

   Purpose

   gene cloning of MR1B

   Depositor

   Gennaro De Libero, Lucia Mori

   Insert

   MR1B cDNA (MR1P1 Human)
   

   Use

   Cloning

   Mutation

   Stop codon has been removed by HindIII site

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. BMPR1B gRNA (BRDN0001145690)

   Plasmid

   #77488

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BMPR1B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   BMPR1B (BMPR1B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. BMPR1B gRNA (BRDN0001149274)

   Plasmid

   #77489

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BMPR1B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   BMPR1B (BMPR1B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. BMPR1B gRNA (BRDN0001148923)

   Plasmid

   #77490

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BMPR1B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   BMPR1B (BMPR1B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. BMPR1B gRNA (BRDN0001148600)

   Plasmid

   #77491

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BMPR1B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   BMPR1B (BMPR1B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_Gal4UAS_Blinatumomab_PGK_mCherry

    Plasmid

    #85437

    Purpose

    [Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    Blinatumomab
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-MT GLI2 FL

    Plasmid

    #17648

    Depositor

    Erich Roessler

    Article

    Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

    Insert

    GLI2 (GLI2 Human)
    

    Tags

    6x Myc

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Kir2.1-T2A-tdTomato

    Plasmid

    #60598

    Purpose

    Expresses Kir2.1-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Kir2.1-T2A-tdTomato (Kcnj2 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid

    #139996

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-hERalpha

    Plasmid

    #101141

    Purpose

    Mammalian expression of full-length human estrogen receptor-alpha

    Depositor

    Elizabeth Wilson

    Article

    Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

    Insert

    estrogen receptor-alpha (ESR1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-HASAP

    Plasmid

    #138325

    Purpose

    chemigenetic voltage indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Insert

    HASAP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-Pur-CAG-EGFP

    Plasmid

    #80945

    Purpose

    human AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid

    #59987

    Purpose

    Eef1a (EF1alpha) promoter driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Eef1a (EF1alpha) -1955/-1

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMV306DIhsp+LuxG13

    Plasmid

    #49999

    Purpose

    Integrase removed to increase stability of the plasmid in mycobacteria

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    Bacterial luciferase operon + G13 promoter
    

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Promoter

    G13 from M. marinum G13 promoter driving luxC

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Joint Modular Cloning (JMC) Toolkit

    Plasmid Kit

    #1000000233

    Purpose

    The Joint Modular Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional parts for assembling plant transformation vectors.

    Depositor

    Daniel Voytas

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-endo-Nb37

    Plasmid

    #205514

    Purpose

    Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-Nb37
    

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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