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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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  5. R777-E206 Hs.PTEN-nostop

    Plasmid
    #70490
    Purpose
    Gateway ORF clone of human PTEN [NM_000314.5] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PTEN (PTEN Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70490-plasmid-map-sequence-id-125939

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  14. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100900-plasmid-map-sequence-id-457690

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Showing: 4041 - 4060 of 174135 results