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Search-and-replace genome editing without double-strand breaks or donor DNA.
Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR
Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
132774pCMV-PE1Prime editing in mammalian cells
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132775pCMV-PE2Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b
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132776pCMV-PE2-P2A-GFPPrime editing in mammalian cells with co-translational GFP expression
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132777pU6-pegRNA-GG-acceptorPrime editing in mammalian cells
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132778pU6-Sp-pegRNA-HEK3_CTT_insS. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing
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135955U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lentiNeuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b
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135956hSynapsin-PE2-Cterminal-lentiNeuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b
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135957pU6-Sp-pegRNA-RNF2_+5GtoTS. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing
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135958pU6-sp-sgRNA-RNF2_+41nickS. pyogenes sgRNA for +41 nick in RNF2
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135959pU6-sp-sgRNA-mDNMT1_nick-lentiS. pyogenes nicking sgRNA for mouse DNMT1
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