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Search-and-replace genome editing without double-strand breaks or donor DNA.
Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR
Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4.
PubMed Article

Plasmids from Article

ID Plasmid  
132774pCMV-PE1
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132775pCMV-PE2
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132776pCMV-PE2-P2A-GFP
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132777pU6-pegRNA-GG-acceptor
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132778pU6-Sp-pegRNA-HEK3_CTT_ins
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135955U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti
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135956hSynapsin-PE2-Cterminal-lenti
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135957pU6-Sp-pegRNA-RNF2_+5GtoT
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135958pU6-sp-sgRNA-RNF2_+41nick
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135959pU6-sp-sgRNA-mDNMT1_nick-lenti
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