pCMV-PE2-P2A-GFP
(Plasmid #132776)

• Purpose: Prime editing in mammalian cells with co-translational GFP expression
• Depositing Lab: David Liu
• Publication: Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4.

Backbone

• Vector backbone: pCMV
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PE2-P2A-GFP
• Alt name: Cas9(H840A)-M-MLVrt(D200N, T306K, W313F, T330P, L603W)-P2A-GFP
• Species: Synthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus
• Insert Size (bp): 7170
• Mutation: See manuscript
• Promoter: CMV
• Tags / Fusion Proteins:
  • SV40 NLS (N terminal on backbone)
  • SV40 NLS (C terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7-forward
• 3′ sequencing primer: BGH-reverse

Resource Information

• Supplemental Documents:
  • pCMV-PE2-P2A-GFP.gb
• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid may run as a dimer.