Visual barcodes for clonal-multiplexing of live microscopy-based assays.
Kaufman T, Nitzan E, Firestein N, Ginzberg MB, Iyengar S, Patel N, Ben-Hamo R, Porat Z, Hunter J, Hilfinger A, Rotter V, Kafri R, Straussman R
Nat Commun. 2022 May 18;13(1):2725. doi: 10.1038/s41467-022-30008-0. PubMed Article
Kaufman T, Nitzan E, Firestein N, Ginzberg MB, Iyengar S, Patel N, Ben-Hamo R, Porat Z, Hunter J, Hilfinger A, Rotter V, Kafri R, Straussman R
Nat Commun. 2022 May 18;13(1):2725. doi: 10.1038/s41467-022-30008-0. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158665 | Barcode backbone | This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination |
| 158666 | mTAGBFP2 WC visual barcode | tagging clonal populations with whole cell mTAGBFP2 fluorescent protein |
| 158667 | mTAGBFP2 NES visual barcode | tagging clonal populations with NES mTAGBFP2 fluorescent protein. Adding NES to mTAGBFP2 WC |
| 158668 | mTAGBFP2 Peroxisome visual barcode | tagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WC |
| 158669 | mTurquoise2 WC visual barcode | tagging clonal populations with whole cell mTurquoise2 fluorescent protein |
| 158670 | mTurquoise2 NES visual barcode | tagging clonal populations with NES mTurquoise2 fluorescent protein. |
| 158671 | mTurquoise2 Peroxisome visual barcode | tagging clonal populations with Peroxisomal mTurquoise2 fluorescent protein. |
| 158672 | acGFP WC visual barcode | tagging clonal populations with whole cell acGFP fluorescent protein |
| 158673 | acGFP NES visual barcode | tagging clonal populations with NES acGFP fluorescent protein. Adding NES to acGFP WC |
| 158674 | acGFP Peroxisome visual barcode | tagging clonal populations with Peroxisomal acGFP fluorescent protein. Adding Peroxisome localization signal to acGFP WC |
| 158675 | EYFP WC visual barcode | tagging clonal populations with whole cell EYFP fluorescent protein |
| 158676 | EYFP NES visual barcode | tagging clonal populations with NES EYFP fluorescent protein. Adding NES to EYFP WC |
| 158677 | EYFP Peroxisome visual barcode | tagging clonal populations with Peroxisomal EYFP fluorescent protein. Adding Peroxisome localization signal to EYFP WC |
| 158678 | TRE reporter backbone | This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry |
| 158681 | HIF-TRE-mStrawberry reporter | This plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSD |
| 158682 | YAP/TAZ-TRE-mStrawberry reporter | This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD |
| 158683 | RAR-TRE-mStrawberry reporter | This plasmid is a RAR pathway reporter. It has a Retinoic Acid Receptor Response Element promoter driving the expression of mStrawberry-pGK-BSD |
| 158684 | p53-TRE-mStrawberry reporter | This plasmid is a p53 pathway reporter. It has two copies of wild-type p53 binding sites promoter driving the expression of mStrawberry-pGK-BSD |
| 158685 | AKT-translocation-mStrawberry reporter | This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD |
| 158686 | ERK-KTR-mStrawberry reporter | This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD |
| 158687 | p38-KTR-mStrawberry reporter | This plasmid is a p38 pathway reporter. It has CMV promoter driving the expression of p38-KTR fused to mStrawberry-pGK-BSD |
| 158688 | PKA-KTR-mStrawberry reporter | This plasmid is a PKA pathway reporter. It has CMV promoter driving the expression of PKA-KTR fused to mStrawberry-pGK-BSD |
| 158689 | JNK-KTR-mStrawberry reporter | This plasmid is a JNK pathway reporter. It has CMV promoter driving the expression of JNK-KTR fused to mStrawberry-pGK-BSD |
| 158690 | Geminin-mStrawberry reporter | This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD |
| 158691 | iRFP-H2A | This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers |