|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11911||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2827
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt namephotoactivatable green fluorescent protein
Insert Size (bp)720
MutationThe PAGFP has four mutations (L64F/T65S/V163A/T203H) compared with the Clontech EGFP. The PAGFP also contains an A206K mutation to disrupt dimerization
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The fluorescent protein encoding region contains one of the three substitutions (A206K) suggested by Dr. Roger Tsien. This mutation disrupts fluorescent protein dimerization even at high concentrations. The mutation does not seem to have much effect on the fluorescent properties of the CFP, GFP and YFP versions, so hopefully it will not affect PAGFP too much. The PAGFP A206K mutant does not display obvious fluorescent differences compared with the original, but please bear in mind that I haven't characterized it to the same extent.
The citation for the A206K mutation in fluorescent proteins is Science 2002 296:913.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRSETA-PAGFP was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 11911 ; http://n2t.net/addgene:11911 ; RRID:Addgene_11911)
For your References section:A photoactivatable GFP for selective photolabeling of proteins and cells. Patterson GH, Lippincott-Schwartz J. Science. 2002 Sep 13. 297(5588):1873-7. 10.1126/science.1074952 PubMed 12228718