pMKO.1 puro Rb shRNA
(Plasmid #10670)

• Depositing Lab: William Hahn
• Publication: Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74.

Backbone

• Vector backbone: pMKO.1
• Backbone size w/o insert (bp): 6700
• Vector type: Mammalian Expression, Retroviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rb shRNA
• Alt name: Rb
• Species: H. sapiens (human)
• Insert Size (bp): 58
• Entrez Gene: RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLXSN 5'
• 3′ sequencing primer: pBABE-3

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Sequences specific for 3'UTR of Rb transcript. forward, 5'-CCGGCAGAGATCGTGTATTGAGATTCTCGAGAATCTCAATACACGATCTCTGTTTTTG-3'; and reverse, 5'-AATTCAAAAACAGAGATCGTGTATTGAGATTCTCGAGAATCTCAATACACGATCTCTG-3'