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Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.
Crawford KHD, Eguia R, Dingens AS, Loes AN, Malone KD, Wolf CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD
Viruses. 2020 May 6;12(5). pii: v12050513. doi: 10.3390/v12050513.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
155130HDM-SARS2-Spike-delta21Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping
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158762HDM_SARS2_Spike_del21_D614GMammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614
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