172,186 results

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Flag-HA-YOD1

Plasmid

#22554

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

YOD1 (YOD1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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TFORF3457

Plasmid

#144933

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

IRF9 (IRF9 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3037

Plasmid

#144513

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

IRF2 (IRF2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSANG10-3F

Plasmid

#39264

Depositor

John McCafferty

Article

Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

Type

Empty backbone

Use

Bacterial expression, bacterial expression of re…

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pDawn

Plasmid

#43796

Purpose

Empty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.

Depositor

Andreas Moeglich

Article

Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

Type

Empty backbone

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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Sfp pet29b C-terminal His Tag

Plasmid

#75015

Purpose

Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

Depositor

Michael Burkart

Article

Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

Insert

Sfp

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-MKK6-EE

Plasmid

#82718

Purpose

Expresses constitutively active human MKK6. Multiple cloning site 2 is empty.

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK6 (MAP2K6 Human)

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pXPR_003 sgMSLN

Plasmid

#193588

Purpose

MSLN knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgMSLN (MSLN Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsTagRFP

Plasmid

#205757

Purpose

Expression of rsTagRFP in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsTagRFP

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-DIO-GCaMP6s

Plasmid

#184284

Purpose

Expresses GCaMP6s in genetically defined neurons expressing Cre recombinase

Depositor

William Wisden

Article

Yu et al Nat Neurosci. 2019 Jan;22(1):106-119. do

Insert

CCaMP6s

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP

Expression

Mammalian

Promoter

human synaptophysin

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV rtTA3 Blast (w756-1)

Plasmid

#26429

Purpose

3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast

Depositor

Eric Campeau

Insert

Tetracycline repressor A3 mutant

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pBabe-puro/HA-FLAG-RBP2

Plasmid

#14802

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pmScarlet-I_peroxisome_C1

Plasmid

#85065

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Peroxisomal targeting sequence

Tags

mScarlet-i

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRAM18dSGK[MCS]

Plasmid

#84684

Purpose

Can be used for the transformation of rickettsia

Depositor

Ulrike Munderloh

Article

Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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EF.STAT3C.Ubc.GFP

Plasmid

#24983

Purpose

3rd generation lentiviral expression of constitutively active Stat3

Depositor

Linzhao Cheng

Article

Hillion et al Cancer Research 68, 10121, December 2008

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

A662C, N664C (constitutively active)

Available Since

May 28, 2010

Availability

Academic Institutions and Nonprofits only

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Human SAM library in lentiSAMv2 backbone

Pooled Library

#1000000078

Purpose

Genome-wide CRISPR gRNA pooled library for activation of human genes

Depositor

Feng Zhang

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pCAG2-EGFP-C-IFT122

Plasmid

#218737

Purpose

Expresses C-terminally EGFP-tagged IFT122 in mammalian cells

Depositor

Kazuhisa Nakayama

Insert

IFT122 (IFT122 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14132 - pAAV-AiE0779m_3xC2-minBG-jGCaMP8m-WPRE3-BGHpA (Alias: CN4132)

Plasmid

#191735

Purpose

AI plasmid ALIAS: AiP14132. Expression of jGCaMP8m in striatal direct pathway medium spiny neurons (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8m

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCXN2-FLAG-AT2R-CFP

Plasmid

#101660

Purpose

Expresses AT2R with FLAG tag and CFP in mammalian cells.

Depositor

Yusuke Ohba

Article

Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

Insert

Angiotensin II type 2 receptor (AGTR2 Human)

Tags

ECFP and FLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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miniTurbo-V5_pRS415

Plasmid

#107168

Purpose

expresses V5-tagged miniTurbo in the yeast cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Yeast

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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