168,493 results
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Plasmid#58935Purposelabels macrophages with mcherryDepositorInsertmpeg1-mcherry
Promotermpeg1Available SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CFP-SaCas9-HF (without sgRNA)
Plasmid#134470PurposeHuman expression vector for SaCas9-HF variant.DepositorInsertStaphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
UseCRISPRTagsSV40NLS-3xFLAGExpressionMammalianMutationR245A, N413A, N419A, R654APromoterCAGAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSK-mU6-Cj-sgRNA-SV40-puro
Plasmid#192128PurposeExpresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance geneDepositorInsertCj-SgRNA scaffold
ExpressionMammalianAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1
Plasmid#27099DepositorInsertα-Tubulin K40 acetyltransferase1 (ATAT1 Human)
TagsGFP, S-tag, and TEV siteExpressionMammalianPromoterEF1aAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-10X-CS1-3'-puro
Plasmid#212011PurposeDeliver gRNA through lentivirus (compatible with 10x capture Sequence 1)DepositorInsertgRNA scaffold
UseLentiviralAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3
Plasmid#90087PurposeExpresses wild type caspase 3 in bacterial cellsDepositorAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6a
Plasmid#207851PurposeMammalian expression of PE6a prime editorDepositorInsertPE6a
TagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationEc48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…Available SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCaRNA1&2
Plasmid#86087PurposeSimultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cellsDepositorInsertRNA1, RNA2
ExpressionPlantPromoter35SAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCaRNA3
Plasmid#86088PurposeExpression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cellsDepositorInsertRNA3
ExpressionPlantPromoter35SAvailable SinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/F-tractin=mStayGold
Plasmid#212019PurposeFor filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertF-tractin
TagsmStayGoldExpressionMammalianPromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCre
Plasmid#236212PurposeArabinose-inducible Cre recombinaseDepositorInsertCre recombinase
ExpressionBacterialPromoteraraBAD promoterAvailable SinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
hRad51(A89E)–Cas9(D10A)
Plasmid#125563PurposeExpresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV delta R8.2
Plasmid#12263PurposeFirst-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.DepositorInsertHIV-1 GAG/POL, Tat and Rev
UseLentiviral; PackagingExpressionMammalianAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pT4-CMV-GFP
Plasmid#117046PurposeOptimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoterDepositorInsertGFP
UseTransposonPromoterCMVAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 MT
Plasmid#78261PurposepsiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertmutated let-7 target site
UseLuciferase; Microrna activityExpressionMammalianMutationnucleotides 2-5 and 10-11 changed from CTCC to GA…Available SinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-MLS-KillerRed
Plasmid#115517PurposeMiddle entry plasmid containing membrane bound KillerRed fluorophoreDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Hygro-PIP-FUCCI
Plasmid#138714PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianPromoterhPGKAvailable SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-KO
Plasmid#17603PurposeMammalian expression of HA tagged ubiquitin carrying zero lysines (K0)DepositorInsertUbiquitin C (UBC Human)
TagsHAExpressionMammalianMutationKO: all lysines mutated to argininesAvailable SinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDB4695
Plasmid#171127PurposeA plasmid containing the codon-optimized OsTIR1-F74A, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.DepositorInsertOsTIR1-F74A
UseOtherMutationnon applicableAvailable SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-SOX2-17-p2a-KLF4
Plasmid#193290Purposeepisomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversionDepositorExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only