161,511 results

1. MZB211

   Plasmid

   #228494

   Purpose

   10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   pol
   

   Use

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Mutation

   A408S

   Promoter

   T7 Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hsyn-DIO-GCaMP6s

   Plasmid

   #184284

   Purpose

   Expresses GCaMP6s in genetically defined neurons expressing Cre recombinase

   Depositor

   William Wisden

   Article

   Yu et al Nat Neurosci. 2019 Jan;22(1):106-119. do

   Insert

   CCaMP6s
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   human synaptophysin

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLSL.R.Ly

   Plasmid

   #227697

   Purpose

   Reengineered geminiviral replicon vector

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-opCas12a-6xNLS

   Plasmid

   #209022

   Purpose

   Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   opCas12a-6xNLS-Flag-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and MYC

   Expression

   Mammalian

   Mutation

   E174R/S542R

   Promoter

   EF-1a

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-CD14

   Plasmid

   #13645

   Depositor

   Doug Golenbock

   Insert

   CD14 (CD14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. phage UbiC NLS HA stdMCP Halo

   Plasmid

   #104098

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   stdMCP-Halo
   

   Use

   Lentiviral

   Tags

   C-terminal Halo-tag, N-terminal NLS, and Two syno…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

   Plasmid

   #89508

   Purpose

   Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

   Insert

   rAPOBEC1-nCas9 (D10A)-UGI-NLS
   

   Use

   Tags

   UGI and rAPOBEC-XTEN

   Expression

   Bacterial

   Mutation

   D10A mutation of Cas9

   Promoter

   T7

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lifeact-3xmNG::HIS3

   Plasmid

   #227596

   Purpose

   Integration vector for expressing Lifeact-3xmNeonGreen

   Depositor

   Bruce Goode

   Article

   Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

   Insert

   Lifeact-3xmNeonGreen::HIS3
   

   Use

   Tags

   3xmNeonGreen

   Expression

   Yeast

   Mutation

   Truncated ABP140 (aa 1-17 only)

   Promoter

   Abp140

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWS3799-gRNA-Csy4-template

   Plasmid

   #182827

   Purpose

   SpCas9 gRNA-Csy4 template

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Insert

   SpCas9 gRNA scaffold + Csy4 sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRISPaint Gene Tagging Kit

    Plasmid Kit

    #1000000086

    Purpose

    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags

    Depositor

    Veit Hornung

    Available since

    Sept. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pDONR223-NTRK1

    Plasmid

    #23891

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NTRK1 (NTRK1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    V263L relative to NM_001012331

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCVpuro-DEST

    Plasmid

    #119745

    Purpose

    Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Puromycin resistance)

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Benitez-Guijarro et al EMBO J. 2018 Aug 1;37(15). pii: embj.201

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CaMKII-PinkyCaMP

    Plasmid

    #232858

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUCDMp10

    Plasmid

    #170461

    Purpose

    EarlyBac insect cell expression system

    Depositor

    Hiro Furukawa

    Article

    Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

    Insert

    New vector
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSF-p15A-trc- YFP

    Plasmid

    #186569

    Purpose

    p15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

    Depositor

    Teresa de Diego Puente

    Article

    Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX303-ZIM3-KRAB-dCas9

    Plasmid

    #154472

    Purpose

    Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDIRECT_22C

    Plasmid

    #91135

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-nEF-Coff/Fon-NpHR3.3-EYFP (AAV8)

    Viral Prep

    #137154-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-NpHR3.3-EYFP (#137154). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-NpHR3.3-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of NpHR3.3-EYFP (inhibited in presence of Cre) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Flp-dependent)

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

19. AAV-mPGK-eGFP (beta-globin intron)

    Plasmid

    #162513

    Purpose

    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

    Depositor

    Bart Nieuwenhuis

    Article

    Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mPGK

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Plasmid

    #180475

    Purpose

    Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    Zebrafish ubb promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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