168,462 results

1. PH-PLCd1-mNGx1

   Plasmid

   #202751

   Purpose

   expresses fluorescent protein-tagged lipid biosensor

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   PLCD1v2(1-170):mNG2 (PLCD1 Human)
   

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 hE47

   Plasmid

   #16059

   Depositor

   Robert Benezra

   Article

   Jen et al Genes Dev. 1992 Aug . 6(8):1466-79.

   Insert

   E47 (TCF3 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVET-IRES-GFP

   Plasmid

   #107139

   Purpose

   A bicistronic lentiviral mammalian expression vector with a multiple cloning site and GFP

   Depositor

   Aki Manninen

   Article

   Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCKmatBC

   Plasmid

   #138587

   Purpose

   matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

   Depositor

   Markus Jeschek

   Article

   Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

   Insert

   bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   matB contains native GUG start codon

   Promoter

   Plac promoter

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLAI-Env

   Plasmid

   #133996

   Purpose

   Env gene from HIV-1 LAI in pcDNA3.1 expression vector

   Depositor

   Himanshu Garg

   Article

   Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

   Insert

   Envelope Glycoprotein
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0612

   Plasmid

   #143054

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RBPJ (RBPJ Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA CUP1p-NuiHA kanMX

   Plasmid

   #131169

   Purpose

   PCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiae

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Tags

   Nui-HA

   Expression

   Yeast

   Available Since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP C3-Lats1

   Plasmid

   #19053

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Large tumor suppressor kinase 1, N mutant (LATS1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   **

   Available Since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-SpyCatcher

   Plasmid

   #223633

   Purpose

   Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiCRISPRv2-ATG5

    Plasmid

    #99573

    Purpose

    Expresses Cas9 with sgRNA targeting Exon 7 of ATG5

    Depositor

    Edward Campbell

    Article

    Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

    Insert

    gRNA targeting ATG5 (ATG5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmiRFP670-N1

    Plasmid

    #79987

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Has Service

    Cloning Grade DNA

    Insert

    miRFP670
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCLX-PGK-hCDK4

    Plasmid

    #114314

    Purpose

    Constitutive expression of the CDK4 protein

    Depositor

    Patrick Salmon

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7TS

    Plasmid

    #17091

    Depositor

    Paul Krieg

    Type

    Empty backbone

    Available Since

    June 5, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-Src

    Plasmid

    #118308

    Purpose

    Expression of mouse Src

    Depositor

    Philip Stork

    Article

    Schmitt et al Mol Cell. 2002 Jan;9(1):85-94.

    Insert

    Src (Src Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH6-MBP-TEV-BirA

    Plasmid

    #179694

    Purpose

    Expresses His6 tagged MBP-BirA fusion protein

    Depositor

    Mark Arbing

    Insert

    BirA
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. mTurquoise2-MapTau

    Plasmid

    #137807

    Purpose

    Visualization of microtubules

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    MapTau
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Str-Ii_VSVG-SBP-EGFP

    Plasmid

    #65300

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV Retrograde)

    Viral Prep

    #100854-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Promoter

    Syn

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

19. tol2-mpx-mCherry

    Plasmid

    #29585

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

    Insert

    mpx-mCherry
    

    Use

    Zebrafish expression

    Available Since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

    Plasmid

    #60903

    Purpose

    Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    Cas9 dead
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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