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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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161,568 results

Showing: 4181 - 4200 of 161568 results

  1. pCS2+ heiCas9

    Plasmid
    #186929
    Purpose
    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS
    Depositor
    Joachim Wittbrodt
    Article
    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:
    Insert
    myc-flexible linker-oNLS-Cas9-oNLS
    Use
    CRISPR
    Tags
    myc-flexible linker-optimizedNLS and optimizedNLS
    Expression
    Mammalian
    Mutation
    Promoter
    sp6
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    186929-plasmid-map-sequence-id-390733

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  6. pET28a-HsSENP1

    Plasmid
    #71465
    Purpose
    Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 1 (SENP1 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pRRlsinPGK_BFP2_WPRE

    Plasmid
    #91787
    Purpose
    Constitutive expression of mTagBFP2 using PGK promoter in a lentiviral backbone.
    Depositor
    Beverly Torok-Storb
    Insert
    mTagBFP2
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91787-plasmid-map-sequence-id-180618

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  8. mCherry-LaminA-C-18

    Plasmid
    #55068
    Purpose
    Localization: Nuclear Envelope, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Lamin A (LMNA Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55068-plasmid-map-sequence-id-200503

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  9. plex.mcs sv40promoter-luc

    Plasmid
    #129429
    Purpose
    Luc expression under SV40 promoter
    Depositor
    Oskar Laur
    Insert
    LUC
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129429-plasmid-map-sequence-id-256107

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  10. TFORF2699

    Plasmid
    #144349
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KMT2B (KMT2B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144349-plasmid-map-sequence-id-408931

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  13. pLenti6.2_mTurquoise2

    Plasmid
    #113723
    Purpose
    Expresses the fluorescent protein mTurquoise2 in a lentiviral backbone
    Depositor
    Vanessa LaPointe
    Insert
    mTurquoise2
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    113723-plasmid-map-sequence-id-218245

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  15. GST-YAP2

    Plasmid
    #24637
    Depositor
    Kunliang Guan
    Insert
    Yap2 (YAP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2010
    Availability
    Academic Institutions and Nonprofits only
    24637-plasmid-map-sequence-id-174857

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  16. pROF148

    Plasmid
    #155330
    Purpose
    Binary vector for expression of the PULSE system under the control of AtUbi10 promoter. It contains a plant selection cassette. 
    Depositor
    Matias Zurbriggen
    Article
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    LB_Tnos-nptII-Pnos_PAtUbi10-SRDX-NLS-EL222-Tnos_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    155330-plasmid-map-sequence-id-311083

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  18. pRJPaph-mChe

    Plasmid
    #67034
    Purpose
    Plasmid for Paph-mCherry integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Article
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Inserts
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    mCherry
    Use
    Conjugative bacterial vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67034-plasmid-map-sequence-id-115547

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Showing: 4181 - 4200 of 161568 results