164,060 results

1. pLVX-TIGHT-CYCLINF-Tet-OFF

   Plasmid

   #32977

   Depositor

   Michele Pagano

   Insert

   CYCLIN F (CCNF Human)
   

   Use

   Lentiviral

   Tags

   Flag and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   P-TIGHT

   Available since

   Feb. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pmStayGold_mStayGold_I3

   Plasmid

   #239339

   Purpose

   Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

   Depositor

   Torsten Wittmann

   Article

   Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

   Insert

   I3-01
   

   Use

   Tags

   mStayGold

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available since

   June 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas9-sgAAVS1-1

   Plasmid

   #129726

   Purpose

   Expressing SpCas9 and sgAAVS1-1

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   SpCas9 and sgAAVS1-1
   

   Use

   CRISPR

   Tags

   P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC18-mini-Tn7T

   Plasmid

   #64954

   Purpose

   mini-Tn7 base vector with transcriptional terminator

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. Tol2-lyzC-Gal4-Vp16-Crys-CFP

   Plasmid

   #97144

   Purpose

   For Gal4/UAS expression system

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   Gal4-Vp16
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMJA284= pSin-Pur-MCS-Puro

   Plasmid

   #116877

   Purpose

   Lentiviral transfer vector containing a multible cloning site

   Depositor

   Marcos Alcocer

   Article

   Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV2-mtIF3 shRNA_TagRFP657

   Plasmid

   #182367

   Purpose

   shRNA targeting mtIF3 mRNA

   Depositor

   Chunghun Lim

   Article

   Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

   Insert

   mtIF3 (Mtif3 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pICH47742::p35S-ePEmax3::tEURb7

   Plasmid

   #227692

   Purpose

   Golden Gate level 1, position 2, ePEmax3 expression cassette.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV-pDisplay-Halo-T2A-EGFP

   Plasmid

   #223498

   Purpose

   Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker

   Depositor

   James Frank

   Insert

   HaloTag and EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGP-CMV-jGCaMP8f

    Plasmid

    #162373

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8f
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CMV-Sa-SauriCas9

    Plasmid

    #135967

    Purpose

    Expresses Sa-SauriCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AICSDP-23:TJP1-mEGFP

    Plasmid

    #87429

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. PB-CRISPR

    Plasmid

    #160047

    Purpose

    For cloning sgRNA to knockout a specific gene in piggybac plasmid

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Sa-SlugCas9

    Plasmid

    #163797

    Purpose

    Expresses Sa-SlugCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEFIRES NQO1 WT

    Plasmid

    #61735

    Purpose

    Expresses WT human NQO1

    Depositor

    Yosef Shaul

    Article

    Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    EF1a

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXs-C3orf17-gfp

    Plasmid

    #70265

    Purpose

    Expresses C3orf17 in mammalian cells with GFP reporter.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    C3orf17 (NEPRO Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

17. TFORF3041

    Plasmid

    #144517

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX15 (SOX15 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pE-SUMO CRYGD

    Plasmid

    #80755

    Purpose

    Expression of human gamma D-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGD (CRYGD Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSEM253 - sgRNA mosTI hygroR

    Plasmid

    #159827

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI hygroR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LT3GEPIR

    Plasmid

    #111177

    Purpose

    Tet-ON miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge