161,601 results

1. pcDNA5 FRT/TO Plk4-mCherry

   Plasmid

   #80269

   Purpose

   mammalian expression plasmid for Plk4-mCherry

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.pU1a-SpCas9

   Plasmid

   #121507

   Purpose

   Expresses codon-optimized SpCas9 in mammalian cells.

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   SpCas9
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR4-myc-nuc (w556-1)

   Plasmid

   #20026

   Purpose

   Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Gateway entry vector

   Tags

   Myc and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_KRAS_p.G12V

   Plasmid

   #81665

   Purpose

   Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   G12V

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry

   Plasmid

   #114322

   Purpose

   Mifepristone-inducible lentiviral backbone for Gateway cloning of Gene insert together with constitutive mCherry expression

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1162_pAAV-HLP-EmGFP-SpA

   Plasmid

   #109313

   Purpose

   Plasmid for AAV EmGFP Mammalian Expression

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Insert

   EmGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. EMTB-mScarletI

   Plasmid

   #137801

   Purpose

   Visualization of microtubules

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   Ensconsin microtubule binding domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-EGFP-Tau E14 P301L

   Plasmid

   #46909

   Purpose

   expresses EGFP tagged Tau E14 P301L in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau E14 P301L (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   P301L AND all 14 S/P or T/P amino acid residues (…

   Promoter

   CMV

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. IRE1 alpha KA-pcDNA3.EGFP

   Plasmid

   #13010

   Depositor

   Fumihiko Urano

   Article

   Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

   Insert

   Inositol Requiring Enzyme-1 (ERN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R4L, K599A

   Promoter

   CMV

   Available since

   Nov. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-Flex-TC66T

    Plasmid

    #48331

    Purpose

    Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVA

    Depositor

    Liqun Luo

    Article

    Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

    Insert

    TVA receptor mCherry fusion with the 66T mutation
    

    Use

    AAV and Cre/Lox

    Tags

    mCherry fusion to TVA receptor

    Expression

    Mammalian

    Mutation

    66T

    Promoter

    CAG

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIP

    Plasmid

    #79009

    Purpose

    For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGs

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-21(+)Tm9D8* TrpB

    Plasmid

    #207517

    Purpose

    Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine

    Depositor

    Thomas Huber

    Article

    Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

    Insert

    Tryptophan synthase β-subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. BirA-Myc_N_MYOD1

    Plasmid

    #167716

    Purpose

    Expresses MYOD1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    MYOD1 (MYOD1 Human)
    

    Use

    Tags

    Myc-BirA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-EglN1-pcDNA3

    Plasmid

    #18963

    Depositor

    William Kaelin

    Article

    Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

    Insert

    Egg laying Nine - 1 (EGLN1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-15b-EK_C122S_His5

    Plasmid

    #49048

    Purpose

    Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

    Depositor

    Hans Brandstetter

    Article

    Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

    Insert

    Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
    

    Use

    Tags

    5xHis

    Expression

    Bacterial

    Mutation

    P92R, E185D, C122S

    Promoter

    T7

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. sUPRa

    Plasmid

    #223242

    Purpose

    sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

    Depositor

    Colin Akerman

    Article

    Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

    Insert

    sUPRa
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Custom

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYZ038

    Plasmid

    #200227

    Purpose

    universal template to build tRNA-gRNA array for multiplex editing CREEPY

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    tRNA-gRNA scaffold
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cyclin E-pBI-EGFP

    Plasmid

    #16654

    Depositor

    Bert Vogelstein

    Article

    Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

    Insert

    Cyclin E (CCNE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-TetOn-mCherry-OAZ1_FS-YFP

    Plasmid

    #232356

    Purpose

    Mammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)

    Depositor

    Ankur Jain

    Article

    Sharma et al bioRxiv [Preprint]. 2024 Nov 17:2024.08.

    Insert

    OAZ1 derived polyamine sensing module (OAZ1 Human)
    

    Use

    Lentiviral

    Tags

    eYFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lentiviral-TOP-dGFP-reporter

    Plasmid

    #14715

    Purpose

    Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

    Depositor

    Tannishtha Reya

    Article

    Reya et al Nature. 2003 May 22. 423(6938):409-14.

    Insert

    LEF-1/TCF-responsive promoter (Ctnnb1 )
    

    Use

    Lentiviral

    Tags

    d2 eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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