166,461 results

1. pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

   Plasmid

   #67981

   Purpose

   Cas9 activity reporter (control) with mCherry and GFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF3290

   Plasmid

   #144766

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CTCF (CTCF Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. p75-RFP

   Plasmid

   #24092

   Depositor

   Moses Chao

   Insert

   p75NTR (NGFR Human)
   

   Use

   Tags

   DSRed

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-NINJ1

   Plasmid

   #201180

   Purpose

   Transient expression of NINJ1 gene in mammalian cells

   Depositor

   Jonathan Kagan

   Article

   Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

   Insert

   Ninjurin 1 (Ninj1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-HA-PD-L1

   Plasmid

   #121492

   Purpose

   Expression of human PD-L1 WT in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   PD-L1 (CD274 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-BbsI-chiRNA

   Plasmid

   #45946

   Purpose

   Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

   Depositor

   Jill Wildonger, Kate O'Connor-Giles, Melissa Harrison

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   U6-BbsI-chiRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Dm-snRNA:U6:96Ab

   Available Since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV8)

   Viral Prep

   #20297-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   EF1a

   Tags

   mCherry (Cre-dependent)

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pFH2.22_mCherry-MS2x8

   Plasmid

   #205537

   Purpose

   Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.GFAP.Cre.WPRE.hGH (AAV PHP.eB)

   Viral Prep

   #105550-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV.GFAP.Cre.WPRE.hGH (#105550). In addition to the viral particles, you will also receive purified pAAV.GFAP.Cre.WPRE.hGH plasmid DNA. GFAP-driven expression of Cre. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   GFAP

   Tags

   None

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

10. pENN.AAV.CAG.tdTomato.WPRE.SV40

    Plasmid

    #105554

    Purpose

    AAV expression of tdTomato from CAG promoter

    Depositor

    James M. Wilson

    Insert

    tdtomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK18sB

    Plasmid

    #177838

    Purpose

    Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

    Depositor

    Christopher Johnson, Gregg Beckham

    Article

    Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

    Type

    Empty backbone

    Use

    Bacterial gene replacement

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLHKI-pGFPN3

    Plasmid

    #21917

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Full length human HKI in pGFP-N3 (HK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is full length human HKI in pGFP-N3

    Promoter

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti CMV Neo DEST (705-1)

    Plasmid

    #17392

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-iSAM

    Plasmid

    #211495

    Purpose

    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

    Depositor

    Lesley Forrester, Antonella Fidanza

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-mCherry Tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, NA, Splicing acceptor, and TREGV promoter

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. eGFP-SUN1

    Plasmid

    #213515

    Purpose

    Expresses human eGFP-SUN1 in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    SUN1 (SUN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SUN1 has a silent mutation on its gene (nucleotid…

    Promoter

    CMV

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLp_3050sNuc

    Plasmid

    #122030

    Purpose

    Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

    Depositor

    Vincent Eijsink, Geir Mathiesen

    Article

    Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

    Insert

    signal peptide Lp_3050
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    sppA

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-pCALM1-EYFP-WPRE-bGHpA

    Plasmid

    #213971

    Purpose

    AAV expression of EYFP from pCALM1 promoter

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    pCALM1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPRv2-sgCTL

    Plasmid

    #234771

    Purpose

    Non Targeting Control

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Non Targeting Control sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_iPE-C_P2A_Csy4

    Plasmid

    #232428

    Purpose

    Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    iPE-C–P2A–Csy4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. human-GFAP-EGFP

    Plasmid

    #169473

    Purpose

    We generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP.

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    EGFP-hGFAP (GFAP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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