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Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains.
Shi J, Wang E, Milazzo JP, Wang Z, Kinney JB, Vakoc CR
Nat Biotechnol. 2015 May 11. doi: 10.1038/nbt.3235.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
65655MSCV_Cas9_puroRetroviral introduction of Cas9 into mammalian cell line.
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65656LRG (Lenti_sgRNA_EFS_GFP)Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.
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Antibodies from Article