Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #65656)


Item Catalog # Description Quantity Price (USD)
Plasmid 65656 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    Lenti virus
  • Backbone manufacturer
    Modified from lentiCRISPRv1 (Addgene Plasmid 49535)
  • Backbone size (bp) 9294
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Promoter U6 promoter-driven sgRNA expression and EFS promoter-driven GFP expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The U6-sgRNA-EFS-GFP vector was derived from the lentiCRISPR-Cas9 plasmid (Addgene: # 49535) by removing the hCas9 cDNA and replacing the Puro cassette with GFP reporter.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LRG (Lenti_sgRNA_EFS_GFP) was a gift from Christopher Vakoc (Addgene plasmid # 65656 ; ; RRID:Addgene_65656)
  • For your References section:

    Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains. Shi J, Wang E, Milazzo JP, Wang Z, Kinney JB, Vakoc CR. Nat Biotechnol. 2015 May 11. doi: 10.1038/nbt.3235. 10.1038/nbt.3235 PubMed 25961408