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MSCV_Cas9_puro
(Plasmid #65655)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65655 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV_puro
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 6200
  • Total vector size (bp) 10475
  • Vector type
    Mammalian Expression, Retroviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Alt name
    S. pyogenes CRISPR-Cas9
  • Species
    Synthetic
  • Insert Size (bp)
    4200
  • Promoter MSSV_LTR
  • Tag / Fusion Protein
    • 5 ' FLAG tag

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    the human Cas9 coding sequence is PCR cloned from lentiCRISPR-Cas9 plasmid (Addgene: # 49535)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV_Cas9_puro was a gift from Christopher Vakoc (Addgene plasmid # 65655 ; http://n2t.net/addgene:65655 ; RRID:Addgene_65655)
  • For your References section:

    Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains. Shi J, Wang E, Milazzo JP, Wang Z, Kinney JB, Vakoc CR. Nat Biotechnol. 2015 May 11. doi: 10.1038/nbt.3235. 10.1038/nbt.3235 PubMed 25961408