PurposeRetrovirus for delivery of one sgRNA (empty, bbsI)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102796||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Retroviral, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Gene/Insert nameU6 sgRNA
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site bbsI (destroyed during cloning)
- 3′ cloning site bbsI (destroyed during cloning)
- 5′ sequencing primer CGATACAAGGCTGTTAGAG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Constructed by gibson assembly of pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP and pMSCV-IRES-mCherry FP (Addgene #52114). The purpose of this plasmid is to verify the cutting of CRISPR and should be used together with pKLV2(gfp)U6sgRNA5(BbsI)-PGKpuroBFP
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP was a gift from Sarah Teichmann (Addgene plasmid # 102796 ; http://n2t.net/addgene:102796 ; RRID:Addgene_102796)
For your References section:Genome-wide CRISPR Screens in T Helper Cells Reveal Pervasive Crosstalk between Activation and Differentiation. Henriksson J, Chen X, Gomes T, Ullah U, Meyer KB, Miragaia R, Duddy G, Pramanik J, Yusa K, Lahesmaa R, Teichmann SA. Cell. 2019 Jan 7. pii: S0092-8674(18)31569-1. doi: 10.1016/j.cell.2018.11.044. 10.1016/j.cell.2018.11.044 PubMed 30639098