pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP
(Plasmid #102796)

• Purpose: Retrovirus for delivery of one sgRNA (empty, bbsI)
• Depositing Lab: Sarah Teichmann
• Publication: Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)31569-1. doi: 10.1016/j.cell.2018.11.044.

Backbone

• Vector backbone: pMSCV
• Vector type: Mammalian Expression, Retroviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: U6 sgRNA
• gRNA/shRNA sequence: Empty
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: bbsI (destroyed during cloning)
• 3′ cloning site: bbsI (destroyed during cloning)
• 5′ sequencing primer: CGATACAAGGCTGTTAGAG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by gibson assembly of pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP and pMSCV-IRES-mCherry FP (Addgene #52114). The purpose of this plasmid is to verify the cutting of CRISPR and should be used together with pKLV2(gfp)U6sgRNA5(BbsI)-PGKpuroBFP