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pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP
(Plasmid #102796)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102796 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    U6 sgRNA
  • gRNA/shRNA sequence
    Empty
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site bbsI (destroyed during cloning)
  • 3′ cloning site bbsI (destroyed during cloning)
  • 5′ sequencing primer CGATACAAGGCTGTTAGAG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by gibson assembly of pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP and pMSCV-IRES-mCherry FP (Addgene #52114). The purpose of this plasmid is to verify the cutting of CRISPR and should be used together with pKLV2(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP was a gift from Sarah Teichmann (Addgene plasmid # 102796 ; http://n2t.net/addgene:102796 ; RRID:Addgene_102796)
  • For your References section:

    Genome-wide CRISPR Screens in T Helper Cells Reveal Pervasive Crosstalk between Activation and Differentiation. Henriksson J, Chen X, Gomes T, Ullah U, Meyer KB, Miragaia R, Duddy G, Pramanik J, Yusa K, Lahesmaa R, Teichmann SA. Cell. 2019 Jan 7. pii: S0092-8674(18)31569-1. doi: 10.1016/j.cell.2018.11.044. 10.1016/j.cell.2018.11.044 PubMed 30639098