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Genome-wide CRISPR Screens in T Helper Cells Reveal Pervasive Crosstalk between Activation and Differentiation.
Henriksson J, Chen X, Gomes T, Ullah U, Meyer KB, Miragaia R, Duddy G, Pramanik J, Yusa K, Lahesmaa R, Teichmann SA
Cell. 2019 Jan 7. pii: S0092-8674(18)31569-1. doi: 10.1016/j.cell.2018.11.044.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
102796pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFPRetrovirus for delivery of one sgRNA (empty, bbsI)
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102797pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFPRetrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR
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102798pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFPRetrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR
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104861pMSCV-U6gRNA(lib)-PGKpuroT2ABFP library
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117263MSCV-Bhlhe40-3xFLAG-IRES-BFPRetroviral overexpression of Bhlhe40 with a 3xFLAG
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117265MSCV-Scara3-IRES-BFPRetroviral overexpression of Scara3
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117266MSCV-Ccdc134-IRES-BFPRetroviral overexpression of Ccdc134
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117268MSCV-Lrrc40-trunc-IRES-BFPRetroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFP
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Recombinant Antibodies from Article