E. coli K-12 MG1655 RARE
PurposeThis E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||61440||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneE. coli K-12 MG1655 genome
- Backbone size w/o insert (bp) 4600000
Modifications to backboneThe following genes were deleted from the E. coli K-12 MG1655 genome: dkgB, yeaE, dkgA,yqhC, yqhD, yjgB, and yahK.
Growth in Bacteria
This strain carries the DE3 lysogen.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:E. coli K-12 MG1655 RARE was a gift from Kristala Prather (Addgene plasmid # 61440)
For your References section:Synthesis and accumulation of aromatic aldehydes in an engineered strain of Escherichia coli. Kunjapur AM, Tarasova Y, Prather KL. J Am Chem Soc. 2014 Aug 20;136(33):11644-54. doi: 10.1021/ja506664a. Epub 2014 Aug 8. 10.1021/ja506664a PubMed 25076127