|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45605||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerM. Koob (University of Wisconsin, Madison)
- Total vector size (bp) 3267
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Growth Strain(s)DH5a lambda pir
- Promoter R6K
- Cloning method Unknown
- 5′ sequencing primer R6K-3'
- 3′ sequencing primer rrnB-T1-term-F (Common Sequencing Primers)
This is a template plasmids for frt-flanked kan cassette. The kan cassette originally came from pCP15.
pKD3 and pKD4 are identical except for the region between the FRT sites, so they create an identical scar that has stop codons in all six reading frames. As drawn in the map, this scar has an idealized ribosome binding site and start codon for downstream (rightward) gene expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKD4 was a gift from Barry L. Wanner (Addgene plasmid # 45605 ; http://n2t.net/addgene:45605 ; RRID:Addgene_45605)
For your References section:One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Datsenko KA, Wanner BL. Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5. 10.1073/pnas.120163297 PubMed 10829079