pKD4
(Plasmid #45605)

• Depositing Lab: Barry L. Wanner
• Publication: Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5.

Backbone

• Vector backbone: pANTSγ
• Backbone manufacturer: M. Koob (University of Wisconsin, Madison)
• Total vector size (bp): 3267
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5a lambda pir
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FRT-kan-FRT
• Promoter: R6K

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: R6K-3'
• 3′ sequencing primer: rrnB-T1-term-F

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a template plasmids for frt-flanked kan cassette. The kan cassette originally came from pCP15.

pKD3 and pKD4 are identical except for the region between the FRT sites, so they create an identical scar that has stop codons in all six reading frames. As drawn in the map, this scar has an idealized ribosome binding site and start codon for downstream (rightward) gene expression.