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PurposeExpresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122969 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepCP20
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameFlp recombinase
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Insert Size (bp)1272
- Promoter pBAD
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD Forward (Common Sequencing Primers)
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made bypCP20 was originally derived from CGSC https://cgsc2.biology.yale.edu/Strain.php?ID=140691
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pBAD-Flp does not have detectable Flp recombinase activity when arabinose is not present in the growth medium
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-Flp was a gift from Lydia Freddolino (Addgene plasmid # 122969 ; http://n2t.net/addgene:122969 ; RRID:Addgene_122969) -
For your References section:
High-Resolution Mapping of the Escherichia coli Chromosome Reveals Positions of High and Low Transcription. Scholz SA, Diao R, Wolfe MB, Fivenson EM, Lin XN, Freddolino PL. Cell Syst. 2019 Mar 27;8(3):212-225.e9. doi: 10.1016/j.cels.2019.02.004. Epub 2019 Mar 20. 10.1016/j.cels.2019.02.004 PubMed 30904377
