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Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
PubMed Article

Plasmids from Article

ID Plasmid  
85405pGH020_sgRNA_G418-GFP
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85406pGH335_MS2-AID*Δ-Hygro
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85407pGH314_wtGFP S65T, Q80H
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85408pGH311_wtGFP S65T
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85409pGH312_wtGFP Q80H
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85410pGH220_wtGFP
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85411pGH045_EGFP
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85412pGH044_mCherry
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85413pGH224_sgRNA_2xMS2_Puro
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85414pGH183_MS2-AIDΔDead-Hygro
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85415pGH156_MS2-AID-Hygro
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85416pGH153_MS2-AIDΔ-Hygro
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85417pGH125_dCas9-Blast
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