Modified Shipping Schedule: Addgene will be closed November 23rd & 24th for the Thanksgiving holiday. Order processing and shipping may be delayed the week of Nov 20 - 24. If you have any questions, please contact us at [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
PubMed Journal

Plasmids from Article

ID Plasmid  
85405pGH020_sgRNA_G418-GFP Add to Cart
85406pGH335_MS2-AID*Δ-Hygro Add to Cart
85407pGH314_wtGFP S65T, Q80H Add to Cart
85408pGH311_wtGFP S65T Add to Cart
85409pGH312_wtGFP Q80H Add to Cart
85410pGH220_wtGFP Add to Cart
85411pGH045_EGFP Add to Cart
85412pGH044_mCherry Add to Cart
85413pGH224_sgRNA_2xMS2_Puro Add to Cart
85414pGH183_MS2-AIDΔDead-Hygro Add to Cart
85415pGH156_MS2-AID-Hygro Add to Cart
85416pGH153_MS2-AIDΔ-Hygro Add to Cart
85417pGH125_dCas9-Blast Add to Cart