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Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
PubMed Journal

Plasmids from Article

ID Plasmid  
85405pGH020_sgRNA_G418-GFP Add to Cart
85406pGH335_MS2-AID*Δ-Hygro Add to Cart
85407pGH314_wtGFP S65T, Q80H Add to Cart
85408pGH311_wtGFP S65T Add to Cart
85409pGH312_wtGFP Q80H Add to Cart
85410pGH220_wtGFP Add to Cart
85411pGH045_EGFP Add to Cart
85412pGH044_mCherry Add to Cart
85413pGH224_sgRNA_2xMS2_Puro Add to Cart
85414pGH183_MS2-AIDΔDead-Hygro Add to Cart
85415pGH156_MS2-AID-Hygro Add to Cart
85416pGH153_MS2-AIDΔ-Hygro Add to Cart
85417pGH125_dCas9-Blast Add to Cart