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Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85405pGH020_sgRNA_G418-GFPhu6 driven sgRNA vector with G418 and GFP selectable markers
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85406pGH335_MS2-AID*Δ-Hygrolentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker
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85407pGH314_wtGFP S65T, Q80Hlentiviral expression vector for wtGFP S65T-Q80H
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85408pGH311_wtGFP S65Tlentiviral expression vector for wtGFP S65T
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85409pGH312_wtGFP Q80Hlentiviral expression vector for wtGFP Q80H
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85410pGH220_wtGFPlentiviral expression vector for wtGFP
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85411pGH045_EGFPlentiviral expression vector for GFP
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85412pGH044_mCherrylentiviral expression vector for mCherry
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85413pGH224_sgRNA_2xMS2_PurohU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker
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85414pGH183_MS2-AIDΔDead-Hygrolentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker
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85415pGH156_MS2-AID-Hygrolentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker
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85416pGH153_MS2-AIDΔ-Hygrolentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker
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85417pGH125_dCas9-Blastlentiviral expression vector for dCas9 with Blasticidin selectable marker
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