This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Jesse Rinehart Lab Plasmids


The Jesse Rinehart Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

Learn more about research in the Jesse Rinehart Lab.

Addgene Alerts

You must login or create an account to subscribe for Addgene Alerts.

Title Authors Publication
Expanding the genetic code of Escherichia coli with phosphoserine. Park HS, Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren CJ, Rinehart J, Soll D Science. 2011 Aug 26;333(6046):1151-4.
Rinehart Lab Phosphoprotein synthesis components (unpublished)
Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion. Heinemann IU, Rovner AJ, Aerni HR, Rogulina S, Cheng L, Olds W, Fischer JT, Soll D, Isaacs FJ, Rinehart J FEBS Lett. 2012 Oct 19;586(20):3716-22. doi: 10.1016/j.febslet.2012.08.031. Epub 2012 Sep 13.
Rinehart Lab Phosphoprotein synthesis components (unpublished)
A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
pCRT7 NT Topo arabinose (unpublished)
ID Kit Hidden Extra Search Info  
1000000020Rinehart & Söll Phosphoprotein Synthesis Kit Add to Cart
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
34623pKD-SepRS-EFSepSepRS, EF-SepBacterial ExpressionExpanding the genetic code of Escherichia coli with phosphoserine. Science. 2011 Aug 26;333(6046):1151-4. pKK223-3 MMP0688 MMP0688 tufB B21_03809 Add to Cart
34624pCAT112TAG-SepTtRNA^SepBacterial ExpressionExpanding the genetic code of Escherichia coli with phosphoserine. Science. 2011 Aug 26;333(6046):1151-4. pACYC184 Add to Cart
34625pMYO127TAG-SepTMyoglobin-127TAGBacterial ExpressionExpanding the genetic code of Escherichia coli with phosphoserine. Science. 2011 Aug 26;333(6046):1151-4. pSepT
  • Tag / Fusion Protein
    • 6xHIS (C terminal on insert)
  • Add to Cart
    34626pMYOMyoglobinBacterial ExpressionExpanding the genetic code of Escherichia coli with phosphoserine. Science. 2011 Aug 26;333(6046):1151-4. pSepT Add to Cart
    34627pMAL-EFSepEF-SepBacterial ExpressionExpanding the genetic code of Escherichia coli with phosphoserine. Science. 2011 Aug 26;333(6046):1151-4. pMAL-c2x tufB B21_03809 Add to Cart
    34928Top10∆serBExpanding the genetic code of Escherichia coli with phosphoserine. Science. 2011 Aug 26;333(6046):1151-4. None Add to Cart
    34929BL21∆serBExpanding the genetic code of Escherichia coli with phosphoserine. Science. 2011 Aug 26;333(6046):1151-4. None Add to Cart
    52053pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFPsfGFP (Synthetic)Bacterial Expression Rinehart Lab Phosphoprotein synthesis components (unpublished) Used in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired. pCRT7/NT-TOPO Add to Cart
    52054pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)SepRS (Other), EF-Sep (Other), 5x tRNA SepBacterial Expression Rinehart Lab Phosphoprotein synthesis components (unpublished) Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep) pKK223-3 Add to Cart
    52055EcAR7NoneEnhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion. FEBS Lett. 2012 Oct 19;586(20):3716-22. doi: 10.1016/j.febslet.2012.08.031. Epub 2012 Sep 13. Strain for use in Rinehart Lab Phosphoprotein synthesis kit None Add to Cart
    53225PCRT7 tetR pLtetO MBP-MEK1 XX AmpMEK1 (Homo sapiens)Bacterial Expression ; phosphoprotein synthesis Rinehart Lab Phosphoprotein synthesis components (unpublished) human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis pCRT7 MAP2K1 CFC3, MAPKK1, MEK1, MKK1, PRKMK1 Add to Cart
    68283SepOTSβ SepRS/EF-Sep, 1xtRNA-Sep (Synthetic)Bacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Phosphoserine orthogonal translation system pKD Add to Cart
    68284SepOTSγSepRS9/EF-Sep21, 1xtRNA-Sep (Synthetic)Bacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Phosphoserine orthogonal translation system pKD Add to Cart
    68285SepOTSδSepRS/EF-Sep, 1xtRNA-Sep-A37 (Synthetic)Bacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Phosphoserine orthogonal translation system pKD Add to Cart
    68286SepOTSεSepRS9/EF-Sep21, 1xtRNA-Sep-A37 (Synthetic)Bacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Phosphoserine orthogonal translation system pKD Add to Cart
    68287SepOTSζSepRS/EF-Sep, 2xtRNA-Sep (Synthetic)Bacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Phosphoserine orthogonal translation system pKD Add to Cart
    68288SepOTSηSepRS9/EF-Sep21, 2xtRNA-Sep (Synthetic)Bacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Phosphoserine orthogonal translation system pKD Add to Cart
    68289SepOTSθSepRS/EF-Sep, 2xtRNA-Sep-A37 (Synthetic)Bacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Phosphoserine orthogonal translation system pKD Add to Cart
    68290SepOTSιSepRS9/EFSep21, 2xtRNA-Sep-A37 (Synthetic)Bacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Phosphoserine orthogonal translation system pKD Add to Cart
    68291SepOTSκSepRS/EF-Sep, 4xtRNA-Sep-A37 (Synthetic)Bacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Phosphoserine orthogonal translation system pKD Add to Cart
    68292SepOTSλ (recommended)SepRS9/EFSep21, 4xtRNA-Sep-A37 (Synthetic)Bacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Phosphoserine orthogonal translation system (preferred variant) pKD Add to Cart
    68294SepOTSνSepRS9/EF-Sep21, 5xtRNA-Sep (Synthetic)Bacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Phosphoserine orthogonal translation system pKD Add to Cart
    68295GFP E17TAGE17TAG GFP (Synthetic)Bacterial ExpressionA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. E17TAG GFP reporter pCRT7/NT-TOPO Add to Cart
    68296GFP S2TAGGFP S2TAG (Synthetic)Bacterial ExpressionA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. S2TAG GFP reporter pCRT7/NT-TOPO Add to Cart
    68297GFP Q157TAGGFP Q157TAG (Synthetic)Bacterial ExpressionA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Q157TAG GFP reporter pCRT7/NT-TOPO Add to Cart
    68298GFP S2TAG/E17TAGGFP S2TAG/E17TAG (Synthetic)Bacterial ExpressionA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. S2TAG/E17TAG GFP reporter pCRT7/NT-TOPO Add to Cart
    68299GFP E17TAG/Q157TAGGFP E17TAG/Q157TAG (Synthetic)Bacterial ExpressionA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. E17/Q157 TAG GFP reporter pCRT7/NT-TOPO Add to Cart
    68300MBP-MEK1MBP-MEK1 (Homo sapiens)Bacterial ExpressionA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. N-terminal MBP tagged human MEK1 gene for bacterial expression pCRT7/NT-TOPO MAP2K1 CFC3, MAPKK1, MEK1, MKK1, PRKMK1 Add to Cart
    68301MBP-MEK1 S218TAGMBP-MEK1 S218TAG (Homo sapiens)Bacterial ExpressionA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression pCRT7/NT-TOPO MAP2K1 CFC3, MAPKK1, MEK1, MKK1, PRKMK1 Add to Cart
    68302MBP-MEK1 S222TAGMBP-MEK1 S222TAG (Homo sapiens)Bacterial ExpressionA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression pCRT7/NT-TOPO MAP2K1 CFC3, MAPKK1, MEK1, MKK1, PRKMK1 Add to Cart
    68305Beta lactamase S68TAGS68TAG beta lactamase (Synthetic)Bacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Beta lactamase S68TAG gene pCRT7/NT-TOPO Add to Cart
    68306Strain: C321.ΔANoneA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Genomically recoded strain of E. coli (no genomic TAG) None Add to Cart
    68307SupD2xtRNA-SupDBacterial Expression, Synthetic BiologyA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. Plasmid encoding tRNA-SupD for serine amber suppression pKD Add to Cart
    69118E17TAG GFP zeo resistanceE17TAG GFP (Synthetic)Bacterial ExpressionA flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. For E17TAG GFP reporter expression in non-zeocin resistant strains pCRT7/NT-TOPO Add to Cart
    73446pCRT7 NT Topo arabinoseBacterial Expression pCRT7 NT Topo arabinose (unpublished) Used for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptides pCRT7/NT-TOPO
  • Tag / Fusion Protein
    • GST (N-terminal), 6xHis (C-terminal)
  • Add to Cart