|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36096||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Modifications to backboneTo create a yeast GFP expression vector (pGOGFP), the PRC1 promoter and GFP coding sequences were cloned into the BamHI site of pRS424.
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)XL1 Blue
Gene/Insert nameFYVE(EEA1) domain
SpeciesH. sapiens (human)
Entrez GeneEEA1 (a.k.a. MST105, MSTP105, ZFYVE2)
- Promoter PRC1
/ Fusion Protein
- GFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unkown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRS424GFP-FYVE(EEA1) was a gift from Scott Emr (Addgene plasmid # 36096 ; http://n2t.net/addgene:36096 ; RRID:Addgene_36096)
For your References section:Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains. Burd CG, Emr SD. Mol Cell. 1998 Jul;2(1):157-62. 10.1016/S1097-2765(00)80125-2 PubMed 9702203
Map uploaded by the depositor.