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(Plasmid #36092)


Item Catalog # Description Quantity Price (USD)
Plasmid 36092 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Modifications to backbone
    To create a yeast GFP expression vector (pGOGFP), the PRC1 promoter and GFP coding sequences were subcloned into the BamHI site of PRS426. To create a PtdIns(4,5)P2-specific FLARE, we fused two tandem copies of the PLCδ1 PH domain (Kavran et al., 1998) to GFP. PCR was used to generate sequences encoding two PLCδ1 PH domains flanked by either BamHI/EcoRI orEcoRI/SalI restriction sites. These PCR products were digested with BamHI/EcoRI orEcoRI/SalI and cloned in-frame into pGO35 (Burd and Emr, 1998), which had been cut with BglII and SalI to create a plasmid harboring a GFP-2×PH(PLCδ) fusion, pRS426GFP-2×PH(PLCδ).
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number


  • Gene/Insert name
    2xPH(PLCdelta) fusion
  • Species
    R. norvegicus (rat)
  • Mutation
    contains aa11-140 of PLCδ (including the PH domain)
  • Promoter PRC1
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Mark Lemmon, University of Pennsylvania School of Medicine

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid contains aa11-140 when compared to GenBank reference sequence NP_058731.1. According to this reference sequence the PH domain is aa17-128. The additional residues surrounding the PH domain were intentionally cloned into this construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS426GFP-2×PH(PLCδ) was a gift from Scott Emr (Addgene plasmid # 36092 ; ; RRID:Addgene_36092)
  • For your References section:

    The yeast synaptojanin-like proteins control the cellular distribution of phosphatidylinositol (4,5)-bisphosphate. Stefan CJ, Audhya A, Emr SD. Mol Biol Cell. 2002 Feb;13(2):542-57. 10.1091/mbc.01-10-0476 PubMed 11854411