Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22853||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backboneP416 GPD
- Backbone size w/o insert (bp) 5780
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Insert Size (bp)475
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lact-C2-GFP-p416 was a gift from Sergio Grinstein (Addgene plasmid # 22853 ; http://n2t.net/addgene:22853 ; RRID:Addgene_22853)
For your References section:Membrane phosphatidylserine regulates surface charge and protein localization. Yeung T, Gilbert GE, Shi J, Silvius J, Kapus A, Grinstein S. Science. 2008 Jan 11. 319(5860):210-3. 10.1126/science.1152066 PubMed 18187657