pRS406-PHO5-GFP-hFAPP1 PH domain
PurposeExpresses GFP-Tagged human FAPP1 PH domain in yeast
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58723||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameFour-phosphate-adaptor protein 1 PH domain
Alt nameFAPP 1
SpeciesH. sapiens (human)
Entrez GenePLEKHA3 (a.k.a. FAPP1)
- Promoter PHO5
/ Fusion Proteins
- GFP (N terminal on backbone)
- Myc (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
PH domain of FAPP1= Methionine 1 - Threonine 98
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRS406-PHO5-GFP-hFAPP1 PH domain was a gift from Tim Levine (Addgene plasmid # 58723 ; http://n2t.net/addgene:58723 ; RRID:Addgene_58723)
For your References section:Targeting of Golgi-specific pleckstrin homology domains involves both PtdIns 4-kinase-dependent and -independent components. Levine TP, Munro S. Curr Biol. 2002 Apr 30;12(9):695-704. S0960982202007790 [pii] PubMed 12007412