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166,461 results

Showing: 4241 - 4260 of 166461 results

  2. pEM047 [T7-assisted CROPseq sgRNA expression vector]

    Plasmid
    #224899
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA
    Depositor
    Thomas Norman
    Article
    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224899-plasmid-map-sequence-id-443624

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  7. pICE-HA-NLS-I-PpoI

    Plasmid
    #46963
    Purpose
    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    NLS-I-PpoI
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TET
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46963-plasmid-map-sequence-id-66854

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  9. pRSET.HaloTag.v7

    Plasmid
    #226197
    Purpose
    bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    226197-plasmid-map-sequence-id-449504

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  13. pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV PHP.eB)

    Viral Prep
    #105547-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    EF1a
    Tags
    EGFP
    Available Since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only

  15. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Article
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196040-plasmid-map-sequence-id-386150

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  20. pEGFPC1 FKBP12

    Plasmid
    #194118
    Purpose
    Expresses EGFP-FKBP12 fusion protein
    Depositor
    Jun Liu
    Insert
    FKBP1A (FKBP1A Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194118-plasmid-map-sequence-id-380739

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Showing: 4241 - 4260 of 166461 results