168,493 results

1. pGD-p19

   Plasmid

   #196326

   Purpose

   35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor

   Depositor

   Zhenghe Li

   Article

   Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

   Insert

   tomato bushy stunt virus p19
   

   Expression

   Plant

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1-FIRE-pHLy

   Plasmid

   #170775

   Purpose

   Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

   Depositor

   Aimee Kao

   Article

   Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and mTFP1

   Expression

   Mammalian

   Mutation

   84 nucleotide Signal Sequence switched to the beg…

   Promoter

   CMV

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-HA-14-3-3 ϵ

   Plasmid

   #48797

   Purpose

   expresses human myc-14-3-3ϵ in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Chen et al Cell. 2003 May 16;113(4):457-68.

   Insert

   YWHAE 14-3-3 ϵ (YWHAE Human)
   

   Tags

   haemagglutinin

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (AAV9)

   Viral Prep

   #100851-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (#100851). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Promoter

   Syn

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

5. DPYD-FLAG

   Plasmid

   #61616

   Purpose

   The human DPYD was used to study the effect of DPYD expression on EMT

   Depositor

   David Sabatini

   Article

   Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

   Insert

   Dihydropyrimidine dehydrogenase (DPYD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-PLK1-N-16

   Plasmid

   #55119

   Purpose

   Localization: Centrosomes, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   PLK1 (PLK1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC_N_STA5B

   Plasmid

   #167800

   Purpose

   Expresses STA5B in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   STA5B (STAT5B Human)
   

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-H3.3(WT)-3AID-HA-2A-mTurquoiseBSD

   Plasmid

   #225701

   Purpose

   Lentiviral expression of AID degron tagged Wildtype H3.3 and mTurquoise fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   H3.3 (WT) (H3-3A, H3-3B Human)
   

   Use

   Lentiviral

   Tags

   3xAID HA and T2A-mTurquoise

   Expression

   Mammalian

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SGLT1(GFP)-MAP17(nb)

   Plasmid

   #216210

   Purpose

   for expression of human GFP-tagged SGLT1-MAP17 nanobody complex in BacMam system

   Depositor

   Lei Chen

   Article

   Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

   Insert

   SGLT1 GFP fusion and MAP17-nanobody fusion (SLC5A2, PDZK1IP1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNatB (pACYCduet-naa20-naa25)

    Plasmid

    #53613

    Purpose

    Allows expression of the fission yeast NatB complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

    Inserts

    naa20 (naa20 Fission Yeast)
    naa25 (naa25 Fission Yeast)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDD120

    Plasmid

    #119879

    Purpose

    Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pConstitutive_Che9c60 and Che9c61
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167935

    Purpose

    Inducible CRISPRi expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    KRAB-dCas9-DHFR
    

    Use

    Lentiviral

    Promoter

    tetON

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. GANLS-DEVD-BNES

    Plasmid

    #50835

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddRFP B
    

    Tags

    NES sequence LALKLAGLDIGS and triplicated NLS seq…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-Duet1_6xHis-TEV-eGFP-P62

    Plasmid

    #190929

    Purpose

    Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    P62 (SQSTM1 Human)
    

    Tags

    EGFP

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTD-NTwinStrep_Km

    Plasmid

    #45938

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Promoter

    Trc (lactose/IPTG inducible)

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-OPTN

    Plasmid

    #171424

    Purpose

    For the purifcation of OPTN protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    OPTN (OPTN Human)
    

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRA108

    Plasmid

    #209341

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mCherry fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLPCX-Cx43-IRES-GFP

    Plasmid

    #65433

    Purpose

    Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFNMB2-msfGFP

    Plasmid

    #113192

    Purpose

    Mobilizable plasmid for tetracycline inducible gene expression in Francisella novicida

    Depositor

    Marek Basler

    Article

    Brodmann et al Front Cell Infect Microbiol. 2018 Aug 31

    Insert

    msfGFP
    

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAVS1-P-CAG-mCh

    Plasmid

    #80492

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    mCherry
    

    Use

    Donor vector (human)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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