mScarlet3: a brilliant and fast-maturing red fluorescent protein.
Gadella TWJ Jr, van Weeren L, Stouthamer J, Hink MA, Wolters AHG, Giepmans BNG, Aumonier S, Dupuy J, Royant A
Nat Methods. 2023 Apr;20(4):541-545. doi: 10.1038/s41592-023-01809-y. Epub 2023 Mar 27. PubMed Article
Gadella TWJ Jr, van Weeren L, Stouthamer J, Hink MA, Wolters AHG, Giepmans BNG, Aumonier S, Dupuy J, Royant A
Nat Methods. 2023 Apr;20(4):541-545. doi: 10.1038/s41592-023-01809-y. Epub 2023 Mar 27. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 189752 | pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3 | Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis |
| 189753 | pmScarlet3_C1 | C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein |
| 189754 | pDx_mScarlet3 | Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein |
| 189755 | pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3 | Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis |
| 189756 | pmScarlet-I3_C1 | C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein |
| 189757 | pDx_mScarlet-I3 | Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein |
| 189758 | pDx_mRuby3-SYFP2 | Dual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysis |
| 189759 | pDx_mCherry-SYFP2 | Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis |
| 189760 | pDx_FusionRed-SYFP2 | Dual expression vector for bacteria and mammalian cells producing FusionRed-SYFP2 fusion protein for FRET analysis |
| 189761 | pDx_mScarlet-I-SYFP2 | Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis |
| 189762 | pDx_mScarlet-SYFP2 | Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis |
| 189763 | pDx_mScarlet-I3-SYFP2 | Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-SYFP2 fusion protein for FRET analysis |
| 189764 | pDx_mScarlet3-SYFP2 | Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis |
| 189765 | pDx_mScarlet-I3-NCwt-SYFP2 | Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysis |
| 189766 | pDx_mScarlet3-NCwt-SYFP2 | Dual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysis |
| 189767 | pLifeAct-mScarlet3_N1 | Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3 |
| 189768 | pmScarlet3-alphaTubulin_C1 | Construct for labelling microtubules in mammalian cells with mScarlet3-alphatubulin |
| 189769 | pmScarlet3-Peroxi_C1 | Construct for labelling peroxisomes in mammalian cells with peroxisomal targeted mScarlet3 |
| 189770 | pEB3-mScarlet3_N1 | Construct for labelling microtubule plus ends in mammalian cells with EB3-mScarlet3 |
| 189771 | pLCK-mScarlet3_C1 | Construct for labelling plasma membranes in mammalian cells using LCK-mScarlet3 |
| 189772 | pER-mScarlet3_N1 | Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3 |
| 189773 | pmScarlet3-Giantin_C1 | Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion |
| 189774 | p4xmts-mScarlet3_N1 | Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3 |
| 189775 | p3xnls-mScarlet3_N1 | Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3 |
| 189776 | pmScarlet3-LaminB_C1 | Construct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusion |
| 189777 | pCyterm-mScarlet-I3_N1 | Construct for testing oligimerization of mScarlet-I3 in mammalian cells by OSER assay |
| 189778 | pCyterm-mScarlet3_N1 | Construct for testing oligimerization of mScarlet3 in mammalian cells by OSER assay |
| 189779 | pCyterm-mScarlet-I3-NCwt_N1 | Construct for testing oligimerization of mScarlet-I3-NCwt in mammalian cells by OSER assay |