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pDx_mScarlet3-NCwt-SYFP2
(Plasmid #189766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189766 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 189766-D50 50 μg of DNA in Tris buffer $495
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DNA 189766-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTriEx-3
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mScarlet3-NCwt-SYFP2
  • Species
    Synthetic
  • Mutation
    encodes the expected internal mutations relative to mScarlet, but the N- and C-termini of the original mScarlet
  • Promoter CMV & Rha
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that mScarlet3-NCwt encodes the expected internal mutations of mScarlet3 relative to mScarlet, but the N- and C-termini of the original mScarlet. See the Supplemental Information for the associated paper for more details.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDx_mScarlet3-NCwt-SYFP2 was a gift from Dorus Gadella (Addgene plasmid # 189766 ; http://n2t.net/addgene:189766 ; RRID:Addgene_189766)

  • For your References section:

    mScarlet3: a brilliant and fast-maturing red fluorescent protein. Gadella TWJ Jr, van Weeren L, Stouthamer J, Hink MA, Wolters AHG, Giepmans BNG, Aumonier S, Dupuy J, Royant A. Nat Methods. 2023 Apr;20(4):541-545. doi: 10.1038/s41592-023-01809-y. Epub 2023 Mar 27. 10.1038/s41592-023-01809-y PubMed 36973546
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