161,601 results

1. pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

   Plasmid

   #183606

   Purpose

   Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   EGFP-TM
   

   Use

   Tags

   HA, Human PDGFRB transmembrane domain, Mouse IgGK…

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Pgk1

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-Tau(T217A)

   Plasmid

   #187025

   Purpose

   Expresses EGFP tagged human 2N4R tau (with T217A mutation) in mammalian cells with CMV promoter

   Depositor

   George Bloom

   Article

   Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

   Insert

   MAPT (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed Threonine 217 to Alanine

   Promoter

   CMV

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbdCas9S

   Plasmid

   #149638

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, empty

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-NT-3/D15A

   Plasmid

   #73035

   Purpose

   Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells

   Depositor

   Pantelis Tsoulfas

   Article

   Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

   Insert

   NT-3/D15A (NTF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Mutated Aspartic acid in position 15 of the matur…

   Promoter

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. UPB-pancreas (UPB7)

   Plasmid

   #180787

   Purpose

   Unique Projection Barcode (UPB7)-Pancreas for Projection-seq

   Depositor

   Rui Chang

   Article

   Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

   Insert

   hM4D(Gi) (partial )
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. UPB-esophagus (UPB5)

   Plasmid

   #180783

   Purpose

   Unique Projection Barcode (UPB5)-Esophagus for Projection-seq

   Depositor

   Rui Chang

   Article

   Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

   Insert

   hM3Dq ( partial ) (CHRM3 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV2-CAG-mGreenLantern-KASH

   Plasmid

   #191094

   Purpose

   To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Pantelis Tsoulfas, Murray Blackmore

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   mGreenLantern-KASH
   

   Use

   AAV and Synthetic Biology

   Tags

   mGreenLantern fused to the KASH domain of Nesprin…

   Expression

   Mammalian

   Mutation

   Optimizzation was done using the Human codon usage

   Promoter

   CMV enhancer and CAG

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. BRD7 (BRD7A-c005)

   Plasmid

   #98245

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BRD7A (BRD7 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00439:Bromodomain 136-223

   Promoter

   T7

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. SiC-V1-Scr

   Plasmid

   #133042

   Purpose

   SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.

   Depositor

   Sriram Neelamegham

   Article

   Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEK39

    Plasmid

    #210599

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
    

    Use

    Tags

    mRuby3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2Bpro

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Diffusive 27kDa (EGFP)

    Plasmid

    #201362

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Diffusive 67kDa

    Plasmid

    #201338

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    EGFP-6PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Diffusive 34kDa

    Plasmid

    #201334

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    EGFP-1PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-Coff/Fon-GCaMP6F (AAV8)

    Viral Prep

    #137124-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-GCaMP6F (#137124). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-GCaMP6F plasmid DNA. EF1a-driven, Flp-dependent expression of GCaMP6f (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    None

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pLV.5’VIM-eGFP-KI

    Plasmid

    #170547

    Purpose

    Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Inserts

    Left Homology arm for VIM knockin
    Right Homology arm for VIM knockin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PKAcs-GGSD5X-GFP

    Plasmid

    #108584

    Purpose

    Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

    Depositor

    Robert Hughes

    Article

    Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

    Insert

    protein kinase A, catalytic subunit
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H87Q and W196R

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTK_Sox10_enh_84_Citrine

    Plasmid

    #130584

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    Sox10_enh_84
    

    Use

    Enhancer reporter

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJIT163-eAFID-3

    Plasmid

    #167360

    Purpose

    For inducing predictable deletions in protoplasts and wheat plants

    Depositor

    Caixia Gao

    Article

    Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

    Insert

    A3Bctd-Cas9-UDG-T2A-AP lyase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AMIGO2-CD4d3+4-bio

    Plasmid

    #73103

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding AMIGO2 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    AMIGO2 (AMIGO2 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMpGWB126

    Plasmid

    #68580

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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