168,321 results

1. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s (AAV5)

   Viral Prep

   #105715-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s (#105715). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s plasmid DNA. EF1a-driven, Cre-dependent GCaMP6s fused to the presynaptic protein synaptophysin. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Rylan Larsen

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

2. pLPCX2-MT1-MMP-eGFP

   Plasmid

   #89819

   Purpose

   Express in MT1-MMP mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   MT1-MMP (MMP14 Human)
   

   Use

   Retroviral

   Tags

   eGFP

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/hChR2(H134R)-EYFP

   Plasmid

   #20940

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSRKBB-empty

   Plasmid

   #59449

   Purpose

   Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.

   Depositor

   Claudia Schmidt-dannert

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   modified lac

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1 hSCN5A

   Plasmid

   #145374

   Purpose

   expresses human Nav1.5 (WT) in mammalian cells

   Depositor

   Hugues Abriel, Robert Kass

   Article

   Rougier et al Am J Physiol Cell Physiol. 2005 Mar;288(

   Insert

   sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRGEB32

   Plasmid

   #63142

   Purpose

   Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Rice snoRNA U3 promoter for gRNA/PTG expression a…

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a-MVPH

   Plasmid

   #235594

   Purpose

   Expresses the MS2-VP64-P65-HSF1 under the control of human EF1a promoter

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Insert

   MS2-VP64-P65-HSF1
   

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1a

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-D1R

   Plasmid

   #89160

   Purpose

   Express vaccinia virus D1R in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D1R
   

   Expression

   Mammalian

   Promoter

   CAG promotor

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-mCherry

   Plasmid

   #114472

   Purpose

   mCherry Control

   Depositor

   Karl Deisseroth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid

    #107941

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a (E174R/S542R/K548R)
    

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R and K548R

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. tet-AID-PcrA M6-UGI-T2A-mTagBFP2-PGK-Puro-T2A-rtTA

    Plasmid

    #221234

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA M6-UGI-T2A-mTagBFP2
    

    Use

    Lentiviral

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE mNeonGreen-Core (HBc from HBV) IRES puro

    Plasmid

    #122202

    Purpose

    Plasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.

    Depositor

    Raphael Gaudin

    Article

    Hallez et al Nat Microbiol. 2019 Apr 1. pii: 10.1038/

    Inserts

    HBc from HBV AYW
    mNeonGreen
    

    Tags

    GGSGGSGGS linker

    Expression

    Mammalian

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Douglas Kim, Loren Looger, GENIE Project

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWM_12x601_30bpLinker

    Plasmid

    #157789

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_30bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV5)

    Viral Prep

    #162379-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

16. pBS-ldhGFP

    Plasmid

    #27170

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    lactate dehydrogenase promoter region fused with modified green fluorescent protein 5
    

    Expression

    Bacterial

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV-EF1a-mCherryLuciferase-W

    Plasmid

    #200101

    Purpose

    Lentiviral vector expressing mCherry fused with Luciferase at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    Luciferase
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-D12L

    Plasmid

    #89161

    Purpose

    Express vaccinia virus D12L in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D12L
    

    Expression

    Mammalian

    Promoter

    CAG promotor

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDV10_pCMV-ADAR1-p150

    Plasmid

    #200405

    Purpose

    Expresses ADAR1 (p150 isoform) constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    ADAR-p150
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Mouse SAM Library in lenti sgRNA(MS2)_puro optimized backbone

    Pooled Library

    #1000000075

    Purpose

    Genome-wide CRISPR gRNA pooled library for activation of mouse genes

    Depositor

    Feng Zhang

    Available Since

    March 17, 2016

    Availability

    Academic Institutions and Nonprofits only