165,840 results

1. pET28a-sfGFP

   Plasmid

   #85492

   Purpose

   pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL1749 (pDonor, R*MmeI)

   Plasmid

   #160733

   Purpose

   Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available Since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

   Plasmid

   #200904

   Purpose

   Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn-Soma-jGCaMP8s (AAV9)

   Viral Prep

   #169256-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from AAV-hSyn-Soma-jGCaMP8s (#169256). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8s plasmid DNA. hSyn-driven expression of soma-targeted calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Promoter

   Tags

   None

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-OptoFGFR1

   Plasmid

   #59776

   Purpose

   Expresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused protein

   Depositor

   Won Do Heo

   Article

   Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

   Insert

   OptoFGFR1 (FGFR1 Human, Mustard Weed)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Beta-2-adrenergic receptor-CFP

   Plasmid

   #55794

   Purpose

   Beta-2-adrenergic receptor tagged with ECFP on carboxyl terminus

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   Beta-2-adrenergic receptor-ECFP (ADRB2 Human, Aequorea victoria)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-VIVIT

   Plasmid

   #11106

   Depositor

   Anjana Rao

   Article

   Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

   Insert

   VIVIT
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRISPi Kit

   Plasmid Kit

   #1000000136

   Purpose

   CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.

   Depositor

   Brigitte Gasser

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pEGFP hsp70

   Plasmid

   #15215

   Depositor

   Lois Greene

   Article

   Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

   Insert

   hsp70 (HSPA1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tol2-BetaActin-SecA5-YFP

    Plasmid

    #105664

    Purpose

    Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

    Depositor

    Qing Deng

    Article

    Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

    Insert

    Secreted Annexin5-EYFP (ANXA5 Human)
    

    Use

    Tol2-gateway

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Beta-Actin

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBHSTC104

    Plasmid

    #124356

    Purpose

    Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin

    Depositor

    Hideo Iwai

    Article

    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

    Insert

    gp41-1 N-intein and C-intein (bicistronic)
    

    Use

    Tags

    CcdA-C (SFAD)

    Expression

    Bacterial

    Mutation

    Promoter

    STC

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRRLsinPGK_IRFP720_WPRE

    Plasmid

    #91785

    Purpose

    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    iRFP720
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBBR-Cas9ΔyqhD-HA500

    Plasmid

    #220369

    Purpose

    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

    Depositor

    Yilan Liu

    Inserts

    SpCas9
    sgRNA for yqhD in P. putida KT2440
    

    Use

    CRISPR; Broad host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-GAL4

    Plasmid

    #24345

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    GAL4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti SYN-FLAG-dCas9-VPR

    Plasmid

    #114196

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SYN

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. plRL19

    Plasmid

    #163634

    Purpose

    L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Insert

    L5 Integrase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mycobacterial Optimized Promoter (MOP) and Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV5)

    Viral Prep

    #100845-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    Syn

    Tags

    None

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pSG5 PPAR alpha

    Plasmid

    #22751

    Depositor

    Bruce Spiegelman

    Insert

    PPARalpha (Ppara Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET16B.Pfu

    Plasmid

    #12509

    Purpose

    Bacterial expression of Pfu DNA polymerase

    Depositor

    Sung-Hou Kim

    Article

    Goldman et al Acta Crystallogr D Biol Crystallogr. 199

    Insert

    Pfu DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y218H, Q462R, F533L, K559I and V683A (mutations w…

    Promoter

    Available Since

    Sept. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pColdI-SBP-eIF4A1

    Plasmid

    #233621

    Purpose

    Expression of SBP-tagged eIF4A1 in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    SBP-eIF4A1 (EIF4A1 Human)
    

    Use

    Tags

    His6-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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