168,321 results

1. pLenti Lifeact-mTagBFP2 PuroR

   Plasmid

   #101893

   Purpose

   Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   mTagBFP2

   Promoter

   CMV immediate early

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCLucf

   Plasmid

   #37328

   Depositor

   John Schiller

   Article

   Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

   Inserts

   Luciferase (luciferase Fly)
   GFP
   

   Expression

   Mammalian

   Promoter

   CMV and SV40

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe-GFP-IKBalpha-wt

   Plasmid

   #15263

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKB alpha (NFKBIA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)

   Plasmid

   #197660

   Purpose

   Expresses GFP under the control of J23100 and BCD2

   Depositor

   Eric Young

   Article

   Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBtet-Pur

   Plasmid

   #60507

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   TCE & RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET MBP GFP LIC cloning vector (MBP-msfGFP)

   Plasmid

   #29750

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), MBP, SiriusGFP, and TEV c…

   Expression

   Bacterial

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMW#2

   Plasmid

   #13349

   Depositor

   Marian Walhout

   Article

   Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

   Insert

   HIS3 reporter
   

   Expression

   Yeast

   Available Since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_nV5-AMOT (p130)

   Plasmid

   #172997

   Purpose

   constitutive expression of V5-tagged AMOT (p130)

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   V5-AMOT (p130) (AMOT Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CamKIIa-jGCaMP8m-WPRE (AAV1)

   Viral Prep

   #176751-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   CamKIIalpha

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

10. pLenti_CMV_FR-GECO1c

    Plasmid

    #224358

    Purpose

    Expresses red fluorescent calcium sensor FR-GECO1c

    Depositor

    Adam Cohen

    Article

    Hayward et al Cell Calcium. 2024 Dec 5;125:102983. doi

    Insert

    FR-GECO1c
    

    Use

    Lentiviral

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHiUGE TurboID-HA donor (all-in-one) ORF0

    Plasmid

    #200383

    Purpose

    Tagging endogenously expressed proteins with TurboID-HA, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid

    #229681

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Halo, ALFA

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET3a aSyn murine

    Plasmid

    #108865

    Purpose

    Expresses murine alpha synuclein

    Depositor

    Gabriele Kaminski Schierle

    Insert

    alpha synuclein (Snca Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. T5-pAc5.1 EGFP-EcoRV

    Plasmid

    #206481

    Purpose

    Schneider cell imaging

    Depositor

    Anne Ephrussi

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Tags

    EGFP

    Expression

    Insect

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-EGFP-RhoA-Q63L

    Plasmid

    #12968

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA constitutively active (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q63L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. psgRNAc

    Plasmid

    #114006

    Purpose

    express sgRNA, p15A, CRM

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Insert

    gRNA
    

    Promoter

    BBa_J23119

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET29a-human histone 4-in-One

    Plasmid

    #223237

    Purpose

    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

    Depositor

    Weixing Zhao

    Inserts

    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    

    Tags

    Dual StrepII, His6, TEV protease site, and VSVG

    Expression

    Bacterial

    Mutation

    A103G

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. DRD2-3xHA/pCDNA3.1

    Plasmid

    #196940

    Purpose

    Plasmid expressing an antigen targeting the D2 dopamine receptor

    Depositor

    James Trimmer

    Insert

    DRD2 (DRD2 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGMC00028 (aka pTS0057)

    Plasmid

    #200832

    Purpose

    AAVS1 guide RNA cloned into the lentiSAM puro backbone

    Depositor

    Raj Chari

    Article

    Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

    Insert

    AAVS1 guide RNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-HA-FbxO11-pcDNA3.1-

    Plasmid

    #52501

    Purpose

    expresses human FbxO11 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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