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(Plasmid #84393)


Item Catalog # Description Quantity Price (USD)
Plasmid 84393 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5100
  • Total vector size (bp) 6000
  • Modifications to backbone
    CMV promotor was removed and replaced by PPRE sequence
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    PPARG response element
  • Alt name
  • Alt name
    ApoCIII promotor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    PPARG (a.k.a. CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma)
  • Promoter PPRE
  • Tag / Fusion Protein
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AseI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer PPRE-AseI
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Geoff Wahl (Addgene plasmid # 11680)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PPRE-H2B-eGFP was a gift from Severine Degrelle & Thierry Fournier (Addgene plasmid # 84393 ; ; RRID:Addgene_84393)
  • For your References section:

    New Transcriptional Reporters to Quantify and Monitor PPARgamma Activity. Degrelle SA, Shoaito H, Fournier T. PPAR Res. 2017;2017:6139107. doi: 10.1155/2017/6139107. Epub 2017 Nov 1. 10.1155/2017/6139107 PubMed 29225614