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PPRE-H2B-eGFP
(Plasmid #84393)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84393 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    H2B-GFP
  • Backbone size w/o insert (bp) 5100
  • Total vector size (bp) 6000
  • Modifications to backbone
    CMV promotor was removed and replaced by PPRE sequence
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPARG response element
  • Alt name
    PPRE
  • Alt name
    ApoCIII promotor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    PPARG (a.k.a. CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma)
  • Promoter PPRE
  • Tag / Fusion Protein
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AseI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer PPRE-AseI
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PPRE-H2B-eGFP was a gift from Severine Degrelle & Thierry Fournier (Addgene plasmid # 84393 ; http://n2t.net/addgene:84393 ; RRID:Addgene_84393)
  • For your References section:

    New Transcriptional Reporters to Quantify and Monitor PPARgamma Activity. Degrelle SA, Shoaito H, Fournier T. PPAR Res. 2017;2017:6139107. doi: 10.1155/2017/6139107. Epub 2017 Nov 1. 10.1155/2017/6139107 PubMed 29225614