163,887 results

1. pET24b-APEX2

   Plasmid

   #111702

   Purpose

   For IPTG-inducible expression of APEX2 in E. coli

   Depositor

   Jessica Seeliger

   Article

   Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

   Insert

   APEX2
   

   Use

   Tags

   6xHis and V5

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEZYflag

   Plasmid

   #18700

   Purpose

   pFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSGDlucV3.0

   Plasmid

   #119760

   Purpose

   dual luciferase with FMDV StopGo sequences flanking MCS

   Depositor

   John Atkins

   Article

   Loughran et al Mol Cell. 2022 Oct 6;82(19):3745-3749.e2

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 and T7

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. c-Flag pcDNA3

   Plasmid

   #20011

   Purpose

   This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

   Depositor

   Stephen Smale

   Article

   Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pscAAV-GFP

   Plasmid

   #32396

   Depositor

   John T Gray

   Article

   Gray et al Methods Mol Biol. 2011;807:25-46.

   Insert

   GFP
   

   Use

   AAV; Adeno-associated virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS598 EF1alpha-EGFPcre

   Plasmid

   #11923

   Purpose

   Expresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.

   Depositor

   Brian Sauer

   Article

   Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

   Insert

   EGFP-cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-Tet-On-shRNA-Control

   Plasmid

   #98398

   Purpose

   Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   non-targeting randomized sequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-SHP2 WT

   Plasmid

   #8381

   Depositor

   Ben Neel

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABEmax-Puro V3

   Plasmid

   #226956

   Purpose

   ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-SMVP-Cas9N

    Plasmid

    #80930

    Purpose

    Expresses Cas9N in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57.1 N-term GFP tTA

    Plasmid

    #107551

    Purpose

    All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

    Depositor

    David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP (optional)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSynapsin- soCoChR-GFP (AAV9)

    Viral Prep

    #107708-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSynapsin- soCoChR-GFP (#107708). In addition to the viral particles, you will also receive purified pAAV-hSynapsin- soCoChR-GFP plasmid DNA. Synapsin-driven somatic CoChR (soCoChR) fused to GFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Promoter

    Syn

    Tags

    GFP

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pFA6a-6xGLY-His-tag-kanMX6

    Plasmid

    #20763

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-HA-ER WT

    Plasmid

    #49498

    Purpose

    Expresses HA-tagged ER wild-type in mammalian cells

    Depositor

    Sarat Chandarlapaty

    Article

    Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. ABE8e-N108Q

    Plasmid

    #194157

    Purpose

    Mammalian expression of ABE8e-N108Q

    Depositor

    Dali Li

    Article

    Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

    Insert

    TadA-8e (N108Q)-nSpCas9
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid

    #50463

    Purpose

    Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pscALPS- SIV-MAC251 vpx

    Plasmid

    #115810

    Purpose

    Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251 Vpx
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-RelA

    Plasmid

    #23255

    Depositor

    Warner Greene

    Article

    Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

    Insert

    p65 (RELA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCX459

    Plasmid

    #229214

    Purpose

    CRISPR array plasmid for MAP1B (Spacers 1-24)

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    CRISPR array plasmid for MAP1B (Spacers 1-24)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-ERKKTRClover

    Plasmid

    #59138

    Purpose

    ORF encoding ERK KTR mClover flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter (MAPK1 Mouse, Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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