pGL3-mArg1 promoter/enhancer -31/-3810
(Plasmid #34571)

• Depositing Lab: Peter Murray
• Publication: Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

Backbone

• Vector backbone: pGL3-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): MACH1
• Growth instructions: Mouse Arg1 reporter constructs cloned into the pGL3 backbone are UNSTABLE when grown at 37 degrees for large-scale preps. We recommend that plasmids be re-transformed and grown overnight at 30 degrees on Amp plates. The next day, scrape the entire plate into 100mL LB-Amp media and grow at 30 degrees (or room temp) for 4-6hr then perform conventional plasmid preps.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Arg1 promoter/enhancer -31/-3810
• Species: M. musculus (mouse)
• Insert Size (bp): 3779
• Entrez Gene: Arg1 (a.k.a. AI, Arg-1, PGIF)
• Tag / Fusion Protein:
  • Firefly Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: RVprimer3

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry