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pGL3-mArg1 promoter/enhancer -31/-3810
(Plasmid #34571)


Item Catalog # Description Quantity Price (USD)
Plasmid 34571 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4818
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Mouse Arg1 reporter constructs cloned into the pGL3 backbone are UNSTABLE when grown at 37 degrees for large-scale preps. We recommend that plasmids be re-transformed and grown overnight at 30 degrees on Amp plates. The next day, scrape the entire plate into 100mL LB-Amp media and grow at 30 degrees (or room temp) for 4-6hr then perform conventional plasmid preps.
  • Copy number


  • Gene/Insert name
    Arg1 promoter/enhancer -31/-3810
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Arg1 (a.k.a. AI, AI256583, Arg-1, PGIF)
  • Tag / Fusion Protein
    • Firefly Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-mArg1 promoter/enhancer -31/-3810 was a gift from Peter Murray (Addgene plasmid # 34571 ; ; RRID:Addgene_34571)
  • For your References section:

    Enhancer-mediated control of macrophage-specific arginase I expression. Pauleau AL, Rutschman R, Lang R, Pernis A, Watowich SS, Murray PJ. J Immunol. 2004 Jun 15;172(12):7565-73. PubMed 15187136