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(Plasmid #35554)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35554 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4267
  • Modifications to backbone
    Plasmid pGL4.32[luc2P/NF-κB-RE/Hygro] (Promega) was modified by removing the 1782-bp XbaI fragment and by replacing the NF-κB promoter.
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    4xStat6 promoter
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
  • Promoter 4xStat6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer RVprimer3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Note: The design of the promoter insert is based on a reporter plasmid, TPU474, previously described by Mikita et al., 1996 [PMID 8816495]
  • Articles Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was generated by replacing the HIndIII(blunt)-AdeI fragment of p2xSTAT6-Luc2P (Addgene plasmid 35486) with the KpnI(blunt)-AdeI fragment of p2xSTAT6-Luc2P. The promoter insert consists of four tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG193.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4xSTAT6-Luc2P was a gift from Axel Nohturfft (Addgene plasmid # 35554 ; ; RRID:Addgene_35554)