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pCMV-STAT6-IRES-Neo
(Plasmid #35482)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35482 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 35482-D50 50 μg of DNA in Tris buffer $495
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DNA 35482-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV-SPORT6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Modifications to backbone
    Vector also contains an IRES-Neo insert
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STAT6
  • Alt name
    Signal transducer and activator of transcription 6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2544
  • GenBank ID
    BC075852.1
  • Entrez Gene
    STAT6 (a.k.a. D12S1644, HIES6, IL-4-STAT, STAT6B, STAT6C)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The precursor to this plasmid was an IMAGE clone that was purchased from Source Bioscience, UK.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To generate pCMV-STAT6-IRES-Neo, the NotI-XbaI fragment of pCMV-Stat6 (IMAGE clone info: clone="MGC:90177 IMAGE:5530399") was replaced with that of pCMV-Fcgamma-RIIa-IRES-neo (Addgene plasmid ID 14948).
Lab ID for pCMV-STAT6-IRES-Neo: pANG194.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-STAT6-IRES-Neo was a gift from Axel Nohturfft (Addgene plasmid # 35482 ; http://n2t.net/addgene:35482 ; RRID:Addgene_35482)
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