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p2xSTAT6-Luc2P
(Plasmid #35486)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35486 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 35486-D50 50 μg of DNA in Tris buffer $495
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DNA 35486-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGL4
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4267
  • Modifications to backbone
    Plasmid pGL4.32[luc2P/NF-κB-RE/Hygro] was modified by removing the hygromycin cassette as a 1782-bp XbaI fragment and by replacing the NF-κB promoter.
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STAT6 promoter
  • Promoter 2x Stat6-c/EBP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer RVprimer3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Note: The design of the promoter insert is based on a reporter plasmid, TPU474, previously described by Mikita et al., 1996 [PMID 8816495]

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

KpnI--BglII fragment of precursor plasmid, containing an NFkappaB promoter, was replaced with a pair of annealed oligonucleotides containing two tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG185.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2xSTAT6-Luc2P was a gift from Axel Nohturfft (Addgene plasmid # 35486 ; http://n2t.net/addgene:35486 ; RRID:Addgene_35486)
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