pGL2-NOS2Promoter-Luciferase
(Plasmid #19296)

• Depositing Lab: Charles Lowenstein
• Publication: Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 90(20):9730-4.

Backbone

• Vector backbone: pGL2-Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5598
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NOS2 Promoter
• Alt name: iNOS
• Alt name: inducible nitric oxide synthase
• Species: M. musculus (mouse)
• Insert Size (bp): 1742
• GenBank ID: L23806
• Entrez Gene: Nos2 (a.k.a. MAC-NOS, NOS-II, Nos-2, Nos2a, i-NOS, iNOS)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry