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Addgene

pGL2-NOS2Promoter-Luciferase
(Plasmid #19296)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19296 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL2-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5598
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NOS2 Promoter
  • Alt name
    iNOS
  • Alt name
    inducible nitric oxide synthase
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1742
  • GenBank ID
    L23806
  • Entrez Gene
    Nos2 (a.k.a. MAC-NOS, NOS-II, Nos-2, Nos2a, i-NOS, iNOS)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2-NOS2Promoter-Luciferase was a gift from Charles Lowenstein (Addgene plasmid # 19296 ; http://n2t.net/addgene:19296 ; RRID:Addgene_19296)
  • For your References section:

    Macrophage nitric oxide synthase gene: two upstream regions mediate induction by interferon gamma and lipopolysaccharide. Lowenstein CJ, Alley EW, Raval P, Snowman AM, Snyder SH, Russell SW, Murphy WJ. Proc Natl Acad Sci U S A. 1993 Oct 15. 90(20):9730-4. 10.1073/pnas.90.20.9730 PubMed 7692452