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pGL3-mArg1 base promoter -31/-2365
(Plasmid #34570)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34570 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Mouse Arg1 reporter constructs cloned into the pGL3 backbone are UNSTABLE when grown at 37 degrees for large-scale preps. We recommend that plasmids be re-transformed and grown overnight at 30 degrees on Amp plates. The next day, scrape the entire plate into 100mL LB-Amp media and grow at 30 degrees (or room temp) for 4-6hr then perform conventional plasmid preps.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Arg1 base promoter -31/-2365
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2334
  • Entrez Gene
    Arg1 (a.k.a. AI, AI256583, Arg-1, PGIF)
  • Tag / Fusion Protein
    • Firefly Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-mArg1 base promoter -31/-2365 was a gift from Peter Murray (Addgene plasmid # 34570 ; http://n2t.net/addgene:34570 ; RRID:Addgene_34570)
  • For your References section:

    Enhancer-mediated control of macrophage-specific arginase I expression. Pauleau AL, Rutschman R, Lang R, Pernis A, Watowich SS, Murray PJ. J Immunol. 2004 Jun 15;172(12):7565-73. PubMed 15187136