198,905 results

1. pSJX016

   Plasmid

   #232324

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119 and Pvan

   Available since

   June 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5_TagBFP_P2AT2A_GFP-nb-KS-FtoG

   Plasmid

   #238275

   Purpose

   For overexpression of TagBFP_P2AT2A_GFP-nb-KS-FtoG

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   TagBFP_P2AT2A_GFP-nb-KS-FtoG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5_TagBFP_P2AT2A_GFP-nb-KS

   Plasmid

   #238274

   Purpose

   For overexpression of TagBFP_P2AT2A_GFP-nb-KS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   TagBFP_P2AT2A_GFP-nb-KS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5_TagBFP_P2AT2A_GFP-nb

   Plasmid

   #238273

   Purpose

   For overexpression of TagBFP_P2AT2A_GFP-nb

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   TagBFP_P2AT2A_GFP-nb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF.DEST51-VPS35-mScarlet

   Plasmid

   #215474

   Purpose

   Expression vector with VPS35 tagged with mScarlet

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   VPS35 (VPS35 Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET22b_msfGFP_stag_KS

   Plasmid

   #237630

   Purpose

   For expression of msfGFP_stag_KS in Ecoli

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   stag_KS
   

   Use

   Tags

   msfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b_msfGFP_stag_KS(FtoG)

   Plasmid

   #237631

   Purpose

   For expression of msfGFP_stag_KS(FtoG) in Ecoli

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   stag_KS(FtoG)
   

   Use

   Tags

   msfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQAT25

   Plasmid

   #223397

   Purpose

   T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-FOXA1-T2A-eGFP

   Plasmid

   #182337

   Purpose

   Expresses human FOXA1 and eGFP via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Forkhead Box A1 (FOXA1 Human)
   

   Use

   Tags

   T2A-eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2343

    Plasmid

    #142931

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MITF (MITF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. ZIKV isolate PRVABC59 NS3 helicase domain

    Plasmid

    #228664

    Purpose

    Bacterial expression of the Zika virus NS3 helicase

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Zika virus NS3 helicase
    

    Use

    Tags

    His-GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pHDR2_mGreenLantern-T2A-SYCP3

    Plasmid

    #222523

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to SYCP3.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330_SYCP3_nterm

    Plasmid

    #222524

    Purpose

    Cas9/sgRNA plasmid for targeting SYCP3

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    Cas9, SYCP3 sgRNA (SYCP3 Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiPGK Puro DEST JNKKTRrsGreenF-E

    Plasmid

    #205764

    Purpose

    Expression of JNK KTR rsGreenF-E under PKG promoter (With Puromycin Resistance)

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    JNK KTR rsGreenF-E
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiCMV Puro DEST ERKKTRrsFastLime

    Plasmid

    #205765

    Purpose

    Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    ERK KTR rsFastLime
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB-cT3G-cERH-STRA8

    Plasmid

    #222585

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of STRA8, with hygromycin resistance

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    STRA8 (STRA8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lck-Halo_pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221900

    Purpose

    Expresses Halo7 targeted to the plasma membrane

    Depositor

    Janelia Integrative Imaging

    Insert

    Lck-Halo7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. RoL10X_pET29b

    Plasmid

    #215427

    Purpose

    Expression construct for RoL10X gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    RoL10X
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. ThcCut1_pET29b

    Plasmid

    #215429

    Purpose

    Expression construct for ThcCut1 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    ThcCut1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. GPR182-DuET

    Plasmid

    #213272

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR182 (GPR182 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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