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beta-arrestin1 mYFP
(Plasmid #36916)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36916 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 zeo
  • Backbone size w/o insert (bp) 5015
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-arrestin1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1900
  • Entrez Gene
    Arrb1 (a.k.a. BARRES)
  • Promoter CMV
  • Tag / Fusion Protein
    • mYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    beta-arrestin1 mYFP was a gift from Robert Lefkowitz (Addgene plasmid # 36916 ; http://n2t.net/addgene:36916 ; RRID:Addgene_36916)
  • For your References section:

    G-protein-coupled receptor kinase specificity for beta-arrestin recruitment to the beta2-adrenergic receptor revealed by fluorescence resonance energy transfer. Violin JD, Ren XR, Lefkowitz RJ. J Biol Chem. 2006 Jul 21;281(29):20577-88. Epub 2006 May 10. 10.1074/jbc.M513605200 PubMed 16687412