Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38260||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Mutationdiglycine linker (replacing terminator codon)
GenBank IDNM_000024.5 NP_000015.1
Entrez GeneADRB2 (a.k.a. ADRB2R, ADRBR, B2AR, BAR, BETA2AR)
- Promoter CMV
/ Fusion Proteins
- signal peptide sequence (MKTIIALSYIFCLVFA) (N terminal on insert)
- Flag (N terminal on insert)
- mCFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The Lefkowitz Lab has confirmed this plasmid contains the human gene, and not the rat version described in the publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:β₂AR-mCFP was a gift from Robert Lefkowitz (Addgene plasmid # 38260 ; http://n2t.net/addgene:38260 ; RRID:Addgene_38260)
For your References section:G-protein-coupled receptor kinase specificity for beta-arrestin recruitment to the beta2-adrenergic receptor revealed by fluorescence resonance energy transfer. Violin JD, Ren XR, Lefkowitz RJ. J Biol Chem. 2006 Jul 21;281(29):20577-88. Epub 2006 May 10. 10.1074/jbc.M513605200 PubMed 16687412