|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36978||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneFUW-LPT2 (tetracycline-inducible)
Backbone manufacturerKong-Jie Kah
Modifications to backboneFUW-LPT2 was modified from FUW-tetO by the Weinberg lab.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
SpeciesH. sapiens (human)
Entrez GeneSOX10 (a.k.a. DOM, PCWH, WS2E, WS4, WS4C)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Human Sox10 cDNA, obtained from Harvard DNA Resource Core, was cloned between EcoRI and BamHI of FUW-LPT2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FUW-Sox10 was a gift from Bob Weinberg (Addgene plasmid # 36978 ; http://n2t.net/addgene:36978 ; RRID:Addgene_36978)
For your References section:Slug and Sox9 cooperatively determine the mammary stem cell state. Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van Oudenaarden A, Weinberg RA. Cell. 2012 Mar 2;148(5):1015-28. 10.1016/j.cell.2012.02.008 PubMed 22385965