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pCMV5-AML1B
(Plasmid #12426)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12426 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AML1B
  • Species
    H. sapiens (human)
  • Entrez Gene
    RUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SphI (unknown if destroyed)
  • 5′ sequencing primer pCEP fwd
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C (Genbank NM_001754.4).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-AML1B was a gift from Scott Hiebert (Addgene plasmid # 12426 ; http://n2t.net/addgene:12426 ; RRID:Addgene_12426)
  • For your References section:

    The t(8;21) fusion protein interferes with AML-1B-dependent transcriptional activation. Meyers S, Lenny N, Hiebert SW. Mol Cell Biol. 1995 Apr . 15(4):1974-82. PubMed 7891692