PurposepCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12426||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4657
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneRUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SphI (unknown if destroyed)
- 5′ sequencing primer pCEP fwd (Common Sequencing Primers)
pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C (Genbank NM_001754.4).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5-AML1B was a gift from Scott Hiebert (Addgene plasmid # 12426 ; http://n2t.net/addgene:12426 ; RRID:Addgene_12426)
For your References section:The t(8;21) fusion protein interferes with AML-1B-dependent transcriptional activation. Meyers S, Lenny N, Hiebert SW. Mol Cell Biol. 1995 Apr . 15(4):1974-82. PubMed 7891692