pcDNA3.1 Flag Runx1 FL (P#1790)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14585||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 Hygro
- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1353
Entrez GeneRunx1 (a.k.a. AM, AML1, CBF-alpha-2, Cbfa, Cbfa2, Pebp, Pebp2a2, Pebpa2b)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
The Runx1 insert in this plasmid has an extra 34 amino acids appended onto the c-terminus. The depositor states that this does not affect plasmid function and the plasmid should behave according to the associated publication.
Using PCR cloning, full-length Runx1 (1-451) was cloned into the HindIII/XhoI sites of pcDNA3.1Hygro. 5' primer has Kozak and Flag epitope-tag sequences. The plasmid was used in western blot and coimmunoprecipitation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1 Flag Runx1 FL (P#1790) was a gift from Matija Peterlin (Addgene plasmid # 14585 ; http://n2t.net/addgene:14585 ; RRID:Addgene_14585)
For your References section:Runx1 binds positive transcription elongation factor b and represses transcriptional elongation by RNA polymerase II: possible mechanism of CD4 silencing. Jiang H, Zhang F, Kurosu T, Peterlin BM. Mol Cell Biol. 2005 Dec . 25(24):10675-83. 10.1128/MCB.25.24.10675-10683.2005 PubMed 16314494