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Addgene

Talin-TS
(Plasmid #83376)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83376 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLPCX
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Talin1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    9162
  • Mutation
    Tension sensor module is inserted between V447 and A448
  • GenBank ID
    21894
  • Entrez Gene
    Tln1 (a.k.a. Tln)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP-F40-tagRFP (tension sensor module)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer LNCX-3
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

XhoI RE site in the vector backbone is disrupted.
F40 refers to the 40-aa peptide (GPGGA)8 derived from the elastic spider silk protein flagelliform

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Talin-TS was a gift from Martin Schwartz (Addgene plasmid # 83376 ; http://n2t.net/addgene:83376 ; RRID:Addgene_83376)
  • For your References section:

    Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity. Kumar A, Ouyang M, Van den Dries K, McGhee EJ, Tanaka K, Anderson MD, Groisman A, Goult BT, Anderson KI, Schwartz MA. J Cell Biol. 2016 May 9;213(3):371-83. doi: 10.1083/jcb.201510012. 10.1083/jcb.201510012 PubMed 27161398