|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26019||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5428
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameVinculin tension sensor
Insert Size (bp)4764
- Cloning method Restriction Enzyme
- 5′ cloning site HINDIII (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH (Common Sequencing Primers)
Note that this vector lacks the SP6 sequence present in pcDNA3.1. There is a small insertion in the vector sequence, upstream of Vinculin. Plasmid function should not be affected.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:VinculinTS was a gift from Martin Schwartz (Addgene plasmid # 26019 ; http://n2t.net/addgene:26019 ; RRID:Addgene_26019)
For your References section:Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA. Nature. 2010 Jul 8. 466(7303):263-6. 10.1038/nature09198 PubMed 20613844