Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9.
Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC
Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
68407PL552Donor empty vector with floxed puromycin resistance gene expression cassette
Loading...
68460AAVS1-Neo-CAG-Flpe-ERT2AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene
Loading...
68461AAVS1-Blasticidin-CAG-Flpe-ERT2AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene
Loading...
68463Cas9 sgRNA vectorCas9 sgRNA vector for cloning
Loading...
68465PAX6 sgRNA2targeting PAX6 gene
Loading...
68466PAX6 sgRNA7targeting PAX6 gene
Loading...
68467PAX6 CKO 2/7 Donor plasmiddonor plasmid for targeting PAX6 locus
Loading...
68468Cas9-2A-Creco-expressing Cas9 and Cre recombinase
Loading...