Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9.
Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC
Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. PubMed Article
Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC
Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68407 | PL552 | Donor empty vector with floxed puromycin resistance gene expression cassette |
| 68460 | AAVS1-Neo-CAG-Flpe-ERT2 | AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene |
| 68461 | AAVS1-Blasticidin-CAG-Flpe-ERT2 | AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene |
| 68463 | Cas9 sgRNA vector | Cas9 sgRNA vector for cloning |
| 68465 | PAX6 sgRNA2 | targeting PAX6 gene |
| 68466 | PAX6 sgRNA7 | targeting PAX6 gene |
| 68467 | PAX6 CKO 2/7 Donor plasmid | donor plasmid for targeting PAX6 locus |
| 68468 | Cas9-2A-Cre | co-expressing Cas9 and Cre recombinase |