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Cas9-2A-Cre
(Plasmid #68468)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68468 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Cas9-2A-GFP
  • Modifications to backbone
    Cas9-2A-Cre was constructed by replacing GFP in the Cas9-2A-GFP (Addgene plasmid #44719) with the Cre cDNA amplified frompCAG-Cre (Addgene plasmid #13775)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9 and Cre recombinase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5288
  • Promoter CAG
  • Tag / Fusion Protein
    • P2A-Cre-Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer ggctctagtgcctctgctaacc
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cas9-2A-Cre was a gift from Su-Chun Zhang (Addgene plasmid # 68468 ; http://n2t.net/addgene:68468 ; RRID:Addgene_68468)
  • For your References section:

    Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. 10.1016/j.stem.2015.06.001 PubMed 26145478