Cas9 sgRNA vector
Purpose(Empty Backbone) Cas9 sgRNA vector for cloning
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68463||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonegRNA cloning vector (Addgene plasmid #41824)
- Backbone size (bp) 3900
Modifications to backboneA previously described chimeric guide RNA expression cassette was ordered as gBlocks and cloned into the gRNA cloning vector (Addgene plasmid #41824). The new sgRNA cloning vector (Cas9 sgRNA vector) includes two BbsI restriction sites for rapid cloning of sgRNA
Vector typeMammalian Expression, CRISPR
- Promoter U6
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Please follow the sgRNA cloning protocol provided by the Su-Chun Zhang laboratory to use this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Cas9 sgRNA vector was a gift from Su-Chun Zhang (Addgene plasmid # 68463 ; http://n2t.net/addgene:68463 ; RRID:Addgene_68463)
For your References section:Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. 10.1016/j.stem.2015.06.001 PubMed 26145478