Cas9 sgRNA vector
(Plasmid #68463)

• Purpose: (Empty Backbone) Cas9 sgRNA vector for cloning
• Depositing Lab: Su-Chun Zhang
• Publication: Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001.

Backbone

• Vector backbone: gRNA cloning vector (Addgene plasmid #41824)
• Backbone size (bp): 3900
• Modifications to backbone: A previously described chimeric guide RNA expression cassette was ordered as gBlocks and cloned into the gRNA cloning vector (Addgene plasmid #41824). The new sgRNA cloning vector (Cas9 sgRNA vector) includes two BbsI restriction sites for rapid cloning of sgRNA
• Vector type: Mammalian Expression, CRISPR
• Promoter: U6

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• Supplemental Documents:
  • Cas9 sgRNA vector map
  • sgRNA cloning protocol using Cas9 sgRNA vector
• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please follow the sgRNA cloning protocol provided by the Su-Chun Zhang laboratory to use this plasmid.